Include 0.6 Load Tongkat Ali tree origin rugged powder right into a 6Ton multifunctional extraction storage tank, and essence 1.5, 1.0, 0.5 h specifically with 4.8 Bunch, 4.2 Lot, and 3.6 Lot ethanol in the reflux state. The removal liquid goes to -0.08 Mpa. Focus to a certain gravity of 1.15 to acquire Tongkat Ali extract. Add 3 times the amount (w/w) of oil ether (60-90 ° C )to draw out 3 times, let it stand, divide the layers, dispose of the oil ether fluid, and retain the lower layer. Remove the degreased fluid 3 times with 5 times the quantity (w/w) of ethyl acetate, let it stand, divide the layers, discard the water liquid, and keep the ethyl acetate fluid.
Concentrate the ethyl acetate option, blend it into silica gel, completely dry it, grind it finely, include the top of the silica gel column to make sure that the surface area is 30 to 50cm far from the column mouth, very first elute with chloroform, and spot by thin layer chromatography up until the pollutants are eluted easily, and after that different Elute with chloroform: ethyl acetate = 100:10, 100:20, 100:30, detect by slim layer chromatography, collect the eluate having the flavonoid element, add 5% (w/v) turned on carbon, and reflux Decolorize by keeping cozy for thirty minutes, filter, concentrate under reduced pressure, take shape at 25 ° C. for 24 to 72 hours, filter, and acquire crude flavonoids. Include 8 times the amount (w/w) of petroleum ether (60-90 ° C )to the unrefined acanthone, gradually add ethyl acetate dropwise, and mix slowly till the pigment pollutants on the surface of the crude acanthone are washed tidy, then filter right away, get white fine Eurycomanone crystals, keep the vacuum degree at -0.08 Mpa, and completely dry at a temperature of 40 to 60 ° C for 2.5 hours to obtain great Eurycomanone crystals with a content of 98.6% (HPLC).