Epigallocatechin Gallate Solubility can inhibit the digestion and absorption of nutrients to achieve weight loss by down-regulating the activity of digestive enzymes. In vivo and in vitro studies have confirmed that catechin can down-regulate the activities of digestive enzymes such as pancreatic α-amylase, glucosidase and glucose transporter. Studies have shown that galloylated catechins such as EGCG can inhibit the absorption of glucose by enterocytes due to the competitive inhibition with Na+glucose cotransporters. EGCG inhibits α-amylase activity through hydrophobic association and hydrogen bond formation with pancreatic α-amylase. Lipids are emulsified, hydrolyzed, and absorbed in the small intestine. Lipid transporters on the apical surface of the small intestine facilitate the transfer of fatty acids and cholesterol into enterocytes. The absorbed lipids are packaged in chylomicrons and secreted into the lymphatic system. Many studies have proved that tea polyphenols can interfere with the lipid emulsification process and inhibit the activity of pancreatic lipase and phospholipase. For example, studies have shown that the intervention of Epigallocatechin Gallate Solubility increased the lipid content in the feces of HFD mice, which indicated that the absorption of lipids was inhibited, and they demonstrated in vitro studies that EGCG dose-dependently inhibited pancreatic lipase in a non-competitive manner activity. Food-derived EGCG can regulate energy metabolism by changing the composition of gut microbiota and affecting the growth of certain species of gut microbiota. By supplementing the high-fat diet of HFD-induced C57BL/6J mice with 4% green tea powder, it was found that green tea intake could effectively reduce the body weight of the mice and reduce the accumulation of triglyceride and cholesterol in the liver, which was correlated with The amount of Akkermansia in the microbiome of the small intestine and/or the amount of total microorganisms in the small intestine correlates. The study found that green tea consumption increased the proportion of bifidobacteria in the human gut microbiota, and the number of bifidobacteria was positively correlated with improved glucose tolerance and enhanced insulin sensitivity. Studies have shown that adding 0.6% EGCG (w/w) to the diet of rats, after feeding for 4 weeks, the starch and protein content in rat feces increased significantly compared with the control group, which indicated that food-derived EGCG intervention has an effect on The absorption of starch and protein, and the relative weight of VAT in rats were reduced. By studying the intestinal microbial composition of rats, it was found that EGCG intervention reduced the number of Clostridium microorganisms and increased the number of Bacteroides.
Many cell-level experiments and animal model studies have shown that Epigallocatechin Gallate Solubility can affect the liver, muscle and fat groups. The health benefits of green tea depend on the biochemical properties and bioavailability of the components in the tea, especially EGCG. Epigallocatechin Gallate Solubility is generally considered to be a strong antioxidant that can effectively scavenge free radicals and inhibit the formation of reactive oxygen species (ROS). However, in the process of in vitro cell culture, it was found that EGCG can automatically oxidize and block mitochondrial electron transfer to produce ROS such as H2O2. ROS can induce cell damage and apoptosis, which can be inhibited by adding superoxide dismutase (SOD) in in vitro culture. Autoxidation of EGCG. In humans and animals, due to the presence of antioxidant enzymes in the tissue and the oxygen partial pressure in the tissue is lower than that in the in vitro culture environment, it is generally believed that EGCG autoxidation is not easy to occur in the tissue. The auto-oxidation phenomenon of EGCG in the in vitro culture environment indicated that EGCG had dual functions as pro-oxidant and antioxidant, and the anti-oxidative and pro-oxidative effects of EGCG could be mediated by its effective concentration. For example, in human lymphocytes cultured in vitro, EGCG at a concentration range of 1-100 μM increased DNA double-strand breaks induced by hydrogen peroxide, but EGCG inhibited DNA breaks at a lower concentration range of 0.01-0.1 μM. The bioavailability of Epigallocatechin Gallate Solubility is also one of the factors affecting the beneficial effects of EGCG. Human and animal model studies have shown that the bioavailability of epicatechin and catechin is greater than that of EGCG. After intragastric administration of 75mg/kg EGCG in mice, the concentration of EGCG in mouse plasma is low, and more than 50% of EGCG is converted to glucose. Exist in the form of glycoside conjugates. In the human body, after ingesting 2-3 cups of green tea per day, the peak level of catechin in plasma is 0.2-0.3 μM, and the peak level of EGCG in plasma can reach 2-9 μM after oral administration of high doses of EGCG. Therefore, in order to realize the beneficial effects of EGCG, EGCG can be isolated and purified from green tea for use in the processing of new drugs and health products.
