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Zhishang Chemical

Deoxyshikonin CAS 43043-74-9

Molecular Formula: C16H16O4

Formula Weight: 272.3

ZSpharmac: Deoxyshikonin Supplier

Product Name: Deoxyshikonin
CAS No: 43043-74-9
Purity: 99%

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Basic Info

Product Name:Deoxyshikonin
Other Names:Deoxyshikonin Reference Substance
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:White Powder
Provide:Deoxyshikonin MSDS;
Deoxyshikonin COA

What is Deoxyshikonin?

The traditional Chinese medicine comfrey is a variety of plants in the genus Anlebiaeuhroma (Royle) Johnst., also known as Xinjiang comfrey), Lithospermumerythrorhizon Sieb. Et Zucc., and Arnebiaguttata Bunge. dry roots. It is mainly distributed in various provinces of our country, and also distributed in some other countries or regions. Comfrey was first recorded in “Shen Nong’s Materia Medica”, which has the effects of clearing away heat and cooling blood, promoting blood circulation, and detoxifying rashes. It is used for blood-heat and toxin-filled, impermeable measles, sores, eczema, water and fire burns, etc. There are two main components of comfrey: one is water-soluble components, which are less studied at present, and it is preliminarily believed to be a mixture of polysaccharides and glycoproteins; the other is a variety of highly fat-soluble substances with biological activity. Including shikonadhiquinones, phenolic acids, alkaloids, phenols and benzoquinones, triterpene acids and sterols, flavonoids, etc. Among them, the shikonaquinone compounds, including shikonin and a series of derivatives, are the important medicinal ingredients in comfrey.

Deoxyshikonin is one of the main active ingredients in comfrey, which belongs to naphthoquinone compounds. Naphthoquinones are the main active ingredients of comfrey, and their content accounts for 3-6.5%. They have a variety of physiological and pharmacological activities, such as healing and anti-inflammatory, anti-bacterial and anti-viral, antipyretic, hemostatic, anti-tumor, liver protection, and hypoglycemic , sedation and immune regulation.

Deoxyshikonin Property

Melting point 91°C
Boiling point 503.7±50.0 °C(Predicted)
density 1.269±0.06 g/cm3(Predicted)
solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
form Powder


Pharmacological Action of Deoxyshikonin

  1. Utilized as thickener, foam improver in shampoos as well as light cleaning agents, as conditioner in man-made fiber along with leather manufacturing. Diethanolamine Sigma reacts with 70% sulfuric acid to dry out and cyclize to create morpholine (ie, 1,4-oxazacyclohexane). Both morpholine along with diethanolamine are intermediates in organic synthesis. For instance, they can be used to produce some optical bleaching reps in the material market. The fat salts of morpholine can be made use of as chemicals. Morpholine can also be utilized to generate the central inhibitor Fulco. dealt with or as a solvent. Diethanolamine is used in logical chemistry as a reagent as well as a set fluid for gas chromatography to uniquely maintain and divide alcohols, diols, amines, pyridines, quinolines, piperazines, thiols, thioethers as well as water.
  2. Diethanolamine Sigma is an essential rust prevention that can be made use of in main home heating central heating boiler water therapy, coolant for car engines, drilling and also lowering oils, as well as other kinds of fueling oil oils. It is additionally used as an absorbent for cleansing acid gas in gas. Made use of as an emulsifier in numerous cosmetics and likewise pharmaceuticals. It is used as a lubricating substance in the fabric market, as a wetting agent, a softener along with different other natural artificial raw materials.
  3. Benefited from as acid absorbent, plasticizer, conditioner, emulsifier, and so on in adhesives. It is likewise used as a soaking up for acid gases (such as hydrogen sulfide, carbon dioxide, and also extra) in oil gas, gas as well as various other gases. It is the raw thing for the synthesis of medicines, pesticides, shade intermediates along with surfactants. It is made use of as an emulsifier for oils and also waxes, as well as a conditioner for natural leather along with artificial fibers under acidic problems. Made use of as a thickener along with foam improver in shampoos in addition to light cleansing representatives. Furthermore utilized as detergent, lubricating substance, brightener and engine piston ash remover.
  4. Made use of as a complexing representative for silver, cadmium, lead, along with zinc plating.
  5. Used as logical reagents, acid gas absorbents, conditioners and lubricating substances, and also in all-natural synthesis.

Industrial Application of Deoxyshikonin

Deoxyshikonin is also a natural plant pigment, ranging in color from orange red to deep red and purple. It can also be used for coloring cosmetics, pharmaceutical coatings, capsules, industrial printing and dyeing, detergents, etc. The various components and structures of shikonaquinone compounds determine their broad spectrum of functions and diversification of uses, and can be used as intermediate raw materials for natural series products in various industries. As a natural pigment, it also has great development and application prospects in cosmetics and printing and dyeing industries.

Content Determination of Deoxyshikonin

1 Instruments and reagents

1. 1 Instrument

Waterse2695 high-performance liquid chromatograph, Waters2998 diode array detector; KQ-250DB numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); electronic balance FR224CN (manufactured by Shanghai Ohaus Instrument Co., Ltd.); electric constant temperature water bath HH. SY11-NY2C (Beijing Changfeng Instrument Co., Ltd.); SHBIII circulating water vacuum pump (Gongyi Yuhua Instrument Co., Ltd.).

1.2 Materials and reagents

Deoxyshikonin reference substance (Shanghai Yuanye Biotechnology Co., Ltd. B21034-10MG); Shandong comfrey, Anhui comfrey and Xinjiang comfrey (purchased in a pharmacy, identified by Duan Rui); acetonitrile chromatographically pure (Tianjin Yongda Reagent Co., Ltd.); Chromatographically pure methanol (Tianjin Yongda Reagent Co., Ltd.).

2 Test methods and results

2.1 Solution preparation

2.1.1 Preparation of reference solution

Accurately weigh 1 mg of the reference substance of Deoxyshikonin, put it in a 10 ml volumetric flask, dissolve it with methanol,

Dilute to the mark and shake well to obtain a reference substance solution with a mass concentration of 0.1 mg/ml, and store in a cool and dry place away from light.

2.1.2 Preparation of the test solution

Grind the dried comfrey, pass through a 60-mesh sieve, accurately weigh 1.0 g of the comfrey powder, and place it in a 50

ml Erlenmeyer flask, add methanol 25 ml, ultrasonically extract for 30 min, shake and let stand for cooling for about 10 min, filter, add 20 ml of methanol to the filter residue, ultrasonically extract for 20 min, combine the methanol filtrate and dilute to 50 ml, and evaporated to dryness in a constant temperature water bath at 40°C, the residue was dissolved in methanol and the volume was adjusted to 25 ml, and filtered through a 0.45 μm microporous membrane to obtain the test solution.

2.2 Methodological investigation and sample determination

2.2.1 Chromatographic conditions and system adaptability test

Chromatographic column: Symmetry C18 BDS column (250 mm×4.6 mm, 5 μm); mobile phase: acetonitrile: water (80:20); flow rate: 1.0 ml/min; detection wavelength: 265 nm; column temperature: 35 ℃; injection volume: 20 μl; detection time: 30min. Under this chromatographic condition, the number of theoretical plates of Deoxyshikonin is greater than 5000, the degree of separation from adjacent chromatographic peaks is greater than 1.7, and the symmetry factor is 1.06.

2.2.2 Specificity test

Get blank solution, reference substance solution, the need testing solution of three production places in right amount, press the sample injection of chromatographic condition under ” 2.2.1 “, record chromatogram (Fig. 1). It can be seen from Figure 1 that the chromatogram of the blank solution has no absorption peak at the corresponding position of the chromatogram of the reference substance, indicating that under the test conditions, other components do not interfere with the measurement results, and the specificity is good.

2. 2.3 Investigation of linear relationship

Precisely measure 1. 0 ml, 2. 0 ml, 3. 0 ml, 4. 0 ml, 5. 0 ml of Deoxyshikonin reference substance solution (0. 1 mg/ml), place them in 10 ml volumetric flasks respectively, add Dilute methanol to the mark, shake well, and prepare 10.0 μg/ml, 20.0 μg/ml, 30.0 μg/ml, 40.0 μg/ml, 50.0 μg/ml solutions, respectively take 20 μl of each solution Samples were analyzed under the above chromatographic conditions. Take the reference substance concentration (μg/ml) as the abscissa (X), and the peak area as the ordinate (Y), draw a standard curve and perform regression calculation to obtain a linear regression equation: Y = 34769X-62579, r = 0.9994 , indicating that Deoxyshikonin has a good linear relationship in the range of 10. 00~50. 00μɡ/ml.

2. 2. 4 Precision test

Take the prepared Deoxyshikonin reference substance solution under the linear relationship item, continuously inject 6 times, record the chromatogram of deoxyshikonin at 265 nm, calculate by peak area, the RSD value is 0.31%, shows that the instrument’s Precision is good.

2.2.5 Stability test

Weigh the comfrey powder from three places of origin, prepare the test solution according to the method under “2.1.2”, and inject samples according to the chromatographic conditions under “2.2.1”, respectively at 0 h, 2 h, 4 h, 6 h, 8 h, 10 h, repeated injection three times in different time periods, each injection 20 μl, recorded the peak area of deoxyshikonin, calculated its content, RSD were 0.10% in Anhui, 0. 07% in Xinjiang and 0. 06% in Shandong, indicating that deoxyshikonin in the three producing areas was relatively stable within 10 hours.

2. 2. 6 Repeatability test

Take comfrey powder from Shandong, Xinjiang, and Anhui respectively, prepare the test solution according to the method under “2.1.2” and process it into 6 parts, inject 20 μl each time, measure 6 times in parallel, and calculate The percentages of deoxyshikonin were 71. 40% in Shandong, 67. 83% in Xinjiang, and 134. 37% in Anhui, and the RSD values were 0. 04%, 0. 10%, and 0. 58%. The method showed good repeatability.

2.2.7 Recovery test

Accurately measure a total of 9 parts of the Anhui test product solution with known content, each 1 ml, and add an appropriate amount of deoxyshikonin reference substance (equivalent to about 80% of the content of deoxyshikonin in Anhui comfrey, 100%, 120%), placed in a 5 ml volumetric flask. The experiment was divided into three groups, each with 3 copies. According to the chromatographic conditions under “2. 2. 1”, inject 20 μl of sample, measure the peak area, and calculate the content of deoxyshikonin. The average sample recovery rates of three different concentrations of low, medium and high concentrations were measured to be 100.17%, 112.82%, and 125.28%, respectively, and the RSD value of 9 sample recovery rate data was 0.10%. within the allowed range.

2.2.8 Content determination of samples

Weigh Shandong, Xinjiang and Anhui comfrey powders respectively, prepare the test solution according to the method under “2.1.2”, process each into 3 parts, and inject samples according to the chromatographic conditions under “2.2.1” , the percentage content of deoxyshikonin in Shandong, Xinjiang and Anhui comfrey were 71. 42%, 67. 77%, 135. 32%, and the RSDs were 0. 02%, 0. 04%, 0. 04%. The results are shown in Table 1. The experimental results show that there are differences in the content of deoxyshikonin in comfrey from different origins.

About Products:
If you need Deoxyshikonin as a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced manufacturer and supplier of Deoxyshikonin, ZSpharmac will provide Deoxyshikonin raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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