D Malic Acid CAS 636-61-3

Bacteria expanded in medium A are inoculated into tool B (bacteria) or tool C (yeast). The microorganisms were incubated at 26 ° C, 220 rpm for 24-hour. Harvested cells were washed from 30 ml of culture in 300 ml flasks with 50 mM phosphate buffer (pH 7.0). The gathered cells were put on hold in 10 ml of pH 7.0-50 mM phosphate buffer containing 0.1% NaCl and 1% DL maleic acid. Breed the big tube including 5 ml of the response mix at 26 ° C, 295 rpm for 48 hr. Finally, the title compound D Malic Acid was gotten by silica gel column chromatography. The artificial route is received Figure 1

Execute the reaction of Medium A containing 10 g DL malic acid, 7 g K2HPO4, 2 g (NH4)2SO4 and 0.1 g MgSO4 7H2O in 1 L of pure water. Before autoclaving, change the pH of the medium to pH 7.2 with NaOH. The response was executed in medium B consisting of 100 g DL disodium maleate, 2 g (NH4)2SO4, 1.4 g KH2PO4, 3.1 g Na2HPO4 12H2O and also 0.25 g MgSO4 7H2O in 1 L of pure water. Prior to autoclaving, the pH of the medium was adjusted to pH 7.2. Grow bacteria in 300 ml Erlenmeyer flasks consisting of 30 ml medium at 26 ° C with rotary trembling (220 r.p.m). A. lwofii ATCC 9036 was cultured in medium A for 7 days. Finally, the title compound D Malic Acid was obtained by silica gel column chromatography. The synthetic path is received Number 1.