Today, although the fermentation method as well as enzymatic method of amino acid production have developed swiftly, and the manufacturing expense has gone down, the protein hydrolysis method of producing amino acid has actually been substantially influenced, but some amino acids are still generated by acid hydrolysis of healthy protein. Amino acids, arginine, leucine, tyrosine, serine and also various other amino acids are still mostly hydrolyzed. Due to the fact that my country is rich in all-natural healthy protein sources, such as human hair, pig hair, waste silk, etc are all excellent basic materials. Cystine 500 is typically prepared from pig hair or human hair basic materials with acid hydrolysis, splitting up and filtration (by isoelectric rainfall).
Hydrolyzed in the hydrolysis tank (tank), put 1000kg of 10mol/L HCl, heated at 70-80℃, then put in washed and dried pig hair or human hair about half the amount of hydrochloric acid (500kg) and continue to heat to 100℃ At the same time, the temperature was adjusted, and the temperature was raised to 110-117°C within 1-1.5h, hydrolyzed for 7h (from 100°C), and the filtrate (hydrolyzate) was collected. The neutralized hydrolyzate was introduced into the neutralization tank, 30%-40% NaOH solution was gradually added and kept stirring, and the pH of the hydrolyzate was adjusted to 4.8 to stop adding alkali. Continue to stir for 15min again, re-measure the pH to be 4.8 to obtain the neutralization solution, leave standstill for 36h, filter to get the precipitate, and centrifugally dry to obtain Cystine 500 crude product I.
Crude, neutralization get Cystine 500 crude product I about 200kg, add the HCl liquid 120kg of 10mol/L, water 480kg (total 800kg), heat dissolving, and keep stirring, when being warming up to 80 ℃, add crude product solution weight 2% activated carbon (about 16kg) was decolorized at 80-90°C for 0.5h, filtered to remove the activated carbon, and the obtained filtrate was adjusted to its pH value with 30% NaOH solution, and kept stirring to make it pH 4.8, and allowed to stand for crystallization. , and then the supernatant liquid was precipitated, and the bottom precipitated crystals were dried to obtain Cystine 500 crude product II.
Refine, neutralize and get above-mentioned Cystine 500 crude product II 50kg, add the HCl solution 250L of 1mol/L, stir and heat to make it dissolve, when being warming up to 70 ℃, add the gac (3kg) of crude product II hydrochloric acid solution weight 1% and heat up Decolorize at 85°C for 0.5h, filter to remove activated carbon, and obtain colorless and transparent filtrate. Then add 1.5 times the weight of distilled water, heat at 75-80 ° C, stir to neutralize with 12% ammonia water to pH 3.5-4.0, suck out the mother liquor above the crystallization while hot, filter the crystallization at the bottom, and use distilled water to wash to remove chlorine-free ions, pump Dry and vacuum dry to obtain Cystine 500.
