Copper Acetate freeze-dried powder preparation enhances skin’s water retention capacity and repairs and regenerates damaged skin, tightens skin and reduces fine lines, and has anti-allergic and anti-inflammatory effects.
CN201811389316.9 provides a blue copper peptide freeze-dried powder preparation, including a solution composed of blue copper peptide freeze-dried powder and Copper Acetate lysozyme solution, wherein the blue copper peptide freeze-dried powder consists of the following mass ratio components Made from:
Tripeptide-1 Copper: 0.5%-1%;
Sodium hyaluronate: 0.02%-0.05%;
The Copper Acetate is also called tripeptide-1 copper;
Copper Acetate in the present invention is a copper peptide and its copper complex, which can be used as an activator and antioxidant for tissue remodeling. It is also a signal peptide that promotes the degradation of a large number of collagen aggregates outside the scar and the synthesis of normal skin collagen , production of elastin, proteoglycans and glucosaminoglycans, growth rate and migration of different cell types, anti-inflammatory, antioxidant responses. The elimination of mannitol in the present invention to peroxynitrite anion is beneficial to skin conditioning and anti-aging, and also has a certain degree of inhibitory effect on skin allergies; mannitol can inhibit the photodamage and photoaging of the skin, and has Moisturizing function. A kind of preparation method of above-mentioned a blue copper peptide freeze-dried powder preparation, including the preparation method of blue copper peptide freeze-dried powder and the preparation method of blue copper peptide freeze-dried powder enzyme solution, wherein the preparation method of blue copper peptide freeze-dried powder Include the following steps:
S1: Selection of raw materials and preparation ratio: select tap water that has been distilled (temperature controlled at 50-70°C) and sterilized, mannitol and Copper Acetate are vacuum-packed before mixing and proportioning, sodium hyaluronate is packaged at a constant temperature ;
S2: Weigh 0.5%-1% blue copper peptide, 5%-15% mannitol, 0.05%-0.2% sodium hyaluronate, 0.2%-0.5% oligopeptide- 1. The remaining amount of deionized water;
S3: Put Copper Acetate, mannitol, sodium hyaluronate and oligopeptide-1 in water, then put the mixture in a stirring device for constant stirring, and stir at a speed of 600r/min for 10min, and stir to form a mixed solution A ;
S4: After the mixed solution A was left to stand for 24 hours, it was again placed in a stirring device for constant speed stirring, and the stirring speed was 1200r/min for 20 minutes;
S5: Filter the mixed solution A through a filter membrane (controlling the diameter to 0.1um), remove the water-insoluble impurities in the mixed solution A to obtain the mixed solution B;
S6: Place the mixed solution B in a centrifugal device for centrifugation, and then perform a pre-freezing operation. At this time, the temperature is controlled at -5°C. After the pre-freezing is completed, the freezing operation is performed. The mixed solution B is sublimated and dried, and the temperature is controlled at -15°C. , the sublimated and dried mixed solution needs to be sterilized by ultraviolet rays.
S7: The mixed solution after sublimation and drying is placed in a sterilized vacuum bag for vacuum packaging, and the storage temperature is controlled below 0°C to obtain powder C
Wherein the preparation method of Copper Acetate freeze-dried powder enzyme solution comprises the following steps:
S1. Weigh sodium hyaluronate, PM-II sclerotin, glycerin, 1,3-butanediol and deionized water according to the mass ratio, stir in a container until transparent, and turn to 7-10Hz with a dispersing disc , then add GF-2TKS pentawin peptide, PGA-WS polyglutamic acid, GC-04 extract, placental protein (animal), essence according to the mass ratio, stir and disperse evenly, turn to 2-5Hz with a dispersing disc , until completely dispersed, sieved through a 200-mesh sieve to prepare Copper Acetate freeze-dried powder enzyme solution D, and powder C was mixed with azurite peptide freeze-dried powder enzyme solution D to obtain a blue copper peptide freeze-dried powder preparation.