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Collagenase CAS 9001-12-1

Molecular Formula: C60H100N2

Formula Weight: 849.4494

ZSpharmac: Collagenase Supplier

Product Name: Collagenase
CAS No: 9001-12-1
Purity: 99%

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Basic Info

Product Name:Collagenase
Other Names:Collagenase Ⅳ
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Light Brown Powder
EINECS No.:232-582-9
Provide:Collagenase MSDS;
Collagenase COA

What is Collagenase?

Collagenase, additionally referred to as collagenase, clostridial peptidase, English name collagenase, is a protease obtained from the fermentation of Clostridium histolyticum. Medical collagenase is refined from the culture liquid of Clostridium histolyticum ATCC21000. This enzyme has a high decomposing activity on the peptide bond of the gly-pro sequence. Because of the breakage of the initial collagen particles, it offers good conditions for the hydrolysis of other proteases, making it have a distinct function of hydrolyzing collagen as well as denaturing collagen.The clinical enzyme made with this enzyme has the capacity to take in natural collagen and likewise denatured collagen, has a strong digestion impact on lethal cells, in addition to can advertise the development of granulation as well as epithelial cells. This product has no effect on globulin as well as fibrin, so it does not influence the regular vein as well as muscular tissue mass cells of the body. It can be utilized for debridement, scab removal, persistent skin ulcers and also bedsores of 2nd and third degree burns.

Collagenase Property

storage temp. -20°C
solubility Soluble in aqueous buffers.
form powder
color light brown


Efficacy of Collagenase

After ingesting collagenase, the human body will absorb enough protease needed, and the excess part will be excreted from the body. After supplementing protease, the local tissues of the body will quickly restore their original functions as if they were newly born. Collagenase can support the body’s adipose tissue and skin elastic structure, and has obvious changing functions. Its improvement effect is so good that it can be compared with the effect of surgery. Has a great firming and lifting effect.

The reason for choosing collagenase is that it has a high level of safety, good compatibility after being absorbed by the human body, the effect is obvious and natural, and its non-biological origin ensures that it will not cause dangerous symptoms such as allergies, and its own substances will participate in the human body The unique enzymatic effect of protease ensures its non-repelling property; the unique helical structure of protease has a lifting effect on aging and sagging skin, and is widely used in the field of beauty; the network physical structure of collagenase ensures that it will not be damaged due to severe Displacement or loss due to movement.

The main application fields of collagenase are industry, beauty and medicine. In the industrial field, leather factories are used more often. Producers use protease to depilate and soften leather products, which not only saves a lot of time, but also improves the working environment of leather factories to a large extent; in medicine field, collagenase has certain medicinal value, and can be used to treat bronchitis, indigestion and gastrointestinal discomfort, vasculitis and oral purification; in terms of beauty, intravenous injection can be used, collagenase can replenish skin moisture, Smoothes wrinkles, fades scars and removes pigmentation, etc. However, excessive use can also cause side effects, which may cause symptoms of digestive system disorders such as diarrhea and abdominal pain; in daily life, collagenase is also closely related to us, it can be used To tenderize meat, clarify wine and degumming silk.

Classification of Collagenase

Presently readily available bacterial collagenases are generally divided right into 4 kinds according to the distinctions in collagenase task: collagenase kind I, type II, type III and kind IV, as well as there are some biases in application: 1) Collagenase I (Type I Collagenase): Consists of a fairly consistent task of different enzymes (including collagenase, caseinase, clostripain, trypsin activity). Generally utilized for the prep work of epithelial cells, liver, lung, fat and adrenal tissue cells; 2) Collagenase II (Kind II Collagenase): has greater clostripain task, typically used for heart, bone, muscle mass, thymus and cartilage material 3) Collagenase III (Type III Collagenase): contains reduced protease activity, and also is commonly utilized in the prep work of bust cells; 4) Collagenase IV (Type IV Collagenase): contains reduced trypsin activity, generally For the prep work of island cells, or cell preparation experiments that need to keep the honesty of the receptor.

How to Use Collagenase

1. Prep Work of Collagenase Stock Option
Add 100 µL of Ca2+ and also Mg2+- consisting of HBSS (Hank’s Well balanced Salt Option, having Ca2+ and also Mg2+) per tube of 100 mg collagenase, vortex gently to liquify it totally, and prepare 1 g/ml (1000 ×) for storage liquid. After that utilize a low-protein-binding 0.22 μm filter membrane layer to filter as well as sterilize, aliquot into small portions, and freeze at -20 ° C at night.

Defrost on ice prior to use, prevent repeated freezing and thawing. The typically utilized concentration for cells as well as cell dispersion is: 0.5-2.5 mg/ml, and also the commonly utilized concentration for cartilage digestion is 1-2mg/ ml. It requires to be figured out according to details speculative conditions or by referring to the matching literature. Maximum functioning focus.

2. Splitting up of cells
1) Utilize a sterilized scalpel or scissors to reduce the cells right into 3-4mm cells pieces;

2) Clean the tissue block several times with HBSS containing Ca2+ and also Mg2+;.

3) Include adequate HBSS containing Ca2+ and Mg2+ to submerge the cells block, and add collagenase to the required functioning focus;.

4) Breed at 37 ° C for 4-18h. Utilizing a horizontal shaker throughout food digestion as well as supplementing digestion with 3mM CaCl2 can enhance digestion performance.

5) The spread cells can be sieved with stainless-steel or nylon mesh and collected for later use. For incompletely dissociated tissues, include a proper amount of fresh collagenase working remedy as well as continue to incubate at 37 ° C;

. 6 )Laundry the gathered cells numerous times with collagenase-free HBSS;.

7) Resuspend the above cells in cell society medium, and also compute the feasible cell density utilizing an automated cell counter or various other techniques.

8) Inoculate cells on a cell culture dish with an ideal cell society medium.

3. Body organ perfusion.
1) Include collagenase to the HBSS consisting of Ca2+ and Mg2+ preheated at 37 ° C, and add 3mM CaCl2 to help enhance the splitting up efficiency;.

2) Perfuse the collagenase functioning remedy right into the equivalent organs according to the enhanced price;.

3) Pass the perfusate recovered in the above procedure with a stainless-steel or nylon mesh filter to separate dissociated cells or little pieces of cells from larger ones, as well as fresh collagenase is needed for incompletely dissociated tissues The working service was more incubated at 37 ° C;.

4) Wash the accumulated cells several times with collagenase-free HBSS;.

5) Resuspend the above cells in cell culture medium, and also compute the feasible cell density utilizing an automated cell counter or various other methods.

6) Inoculate cells on a cell society meal with an appropriate cell society medium.

About Products:
If you need Collagenase, a cosmetic raw material, please contact our service staff directly, and we will provide you with high-quality products at the best price.
As an experienced Collagenase manufacturer and supplier, ZSpharmac will provide Collagenase raw materials for customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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