Report one
Su Bingfeng, Department of Cardiovascular Medicine, Taizhou Municipal Hospital, and others discussed the effect of Chrysin Supplement on improving cardiac function in rats with acute myocardial infarction and its effect on the expression of matrix metalloproteinase 2 (MMP-2)/mitogen-activated protein kinase (MAPK). Methods: 100 SD rats were randomly divided into 5 groups: normal control group, model group, positive control group (perindopril, 0.4 mg·kg-1), chrysin low-dose and high-dose groups (20, 40 mg·kg-1). kg-1), 20 rats in each group. Except for the normal control group, acute myocardial infarction models were established in the other groups, the positive control group, chrysin low-dose and high-dose groups were given intragastric administration of corresponding drugs after successful modeling, and the normal control group and model group were given equal volumes of normal saline, qd , Continuous administration for 2 weeks. After the experiment, changes in myocardial pathological structure were observed, routine cardiac function indexes and myocardial enzyme indexes were measured, and myocardial MMP-2 and MAPK were measured by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) and western blot. mRNA and protein levels. Results: Compared with the model group, the range of myocardial infarction in the perindopril group and the high-dose chrysin group was significantly smaller, the infarction focus and scar tissue were significantly reduced, and the infiltration of inflammatory cells was reduced; Fibroblasts and inflammatory cell infiltration were evident. Compared with the normal control group, the left ventricular end-diastolic internal diameter (LVIDd), left ventricular end-systolic internal diameter (LVIDs), creatine kinase (CK), creatine kinase isoenzyme (CK-MB), lactate dehydrogenation, and Enzyme (LDH), myocardial MMP-2, MAPK mRNA and protein expressions increased, and fractional shortening (FS) and ejection fraction (EF) levels decreased (P<0.05); compared with the model group, the perindopril group , LVIDd, LVIDs, CK, CK-MB, LDH, myocardial MMP-2, MAPK mRNA and protein expression decreased, FS, EF levels increased in chrysin low-dose group and chrysin high-dose group (P<0.05); Compared with the perindopril group, the chrysin low-dose group had statistically significant differences (P<0.05). Conclusion: Chrysin can significantly improve the cardiac function of rats with acute myocardial infarction, which may be related to the inhibition of myocardial MMP-2 expression by chrysin and the activation of MAPK signaling pathway.
Report two
Lin Qi, Department of Urology, Taizhou Central Hospital, and others explored the effect of chrysin on the invasion and migration of prostate cancer PC-3 cell line by regulating the Wnt/β-catenin (β-catenin) pathway. Methods: PC-3 cell group, 5-fluorouracil group, low-dose chrysin group and high-dose chrysin group were set up. In the 5-fluorouracil group, 5-fluorouracil was added to make the final concentration 80.0 μg/mL; in the chrysin low-dose and high-dose groups, Chrysin Supplement was added to make the final concentration 60.0 μg/mL and 120.0 μg/mL, respectively, and cultured for 72 h. Tetramethylazolium salt microenzyme reaction colorimetry (MTT) and crystal violet staining were used to determine the cell survival rate and the number of clones formed, the Transwell method was used to determine the level of cell invasion, and the annexinV-FITC/PI kit was used to determine the level of cell apoptosis. The levels of β-catenin gene and protein in cells were measured by RT-PCR and Western blot. Results: Compared with the PC-3 cell group, the cell survival rate, the number of colony formation, the number of membrane penetration, the expression levels of β-catenin mRNA and protein in the other three groups were all decreased (P<0.05), and the apoptosis rate was increased (P<0.05 ). Compared with the low-dose chrysin group, the high-dose chrysin group had decreased cells, survival rate, colony formation number, membrane penetration number, β-catenin mRNA and protein expression levels (P<0.05), and increased apoptosis rate (P<0.05 ). Compared with the 5-fluorouracil group, the low-dose chrysin group had increased cells, survival rate, cell clone formation number, membrane penetration number, β-catenin mRNA and protein expression levels (P<0.05), and decreased apoptosis rate (P<0.05 ); in the high-dose chrysinin group, the cells, survival rate, cell clone formation number, membrane penetration number, β-catenin mRNA and protein expression levels were decreased (P<0.05), and the apoptosis rate was increased (P<0.05). Conclusion: Chrysin can inhibit the proliferation and invasion of prostate cancer PC-3 cell line, and induce the apoptosis of prostate cancer PC-3 cell line. related to catenin pathway.
