The method for the colorimetric detection of Cetylpyridinium Chloride Covid by copolymerizing column 5 aromatic hydrocarbons in CHCl3 system is as follows:
Step 1. Synthesis of Copolymerized Pillar 5 Aromatics (Compound DCP5-16)
1. Synthesis of intermediates: hydroquinone (4.4g, 40mmol), sodium hydroxide (10g, 4mmol), potassium iodide (4g, 24mmol), hexadecane bromide (26.8g, 88mmol) and ethanol (300.0 mL) into a 500mL round-bottomed flask, stirred and reacted for 3 days (72h), the solid precipitated out to obtain an intermediate. 1HNMR (400MHz, CDCl3, 298K) δ (ppm): 6.82 (d, 4H), 3.96 (t, 4H), 1.75 (t, 4H), 1.43 (t, 4H), 1.26 (t, 48H), 0.86 ( s,6H).. The yield was 80%.
2. Synthesis of aromatics in copolymerization column 5: intermediate (2.79g, 5mmol), p-xylylene dimethyl ether (2.76g, 20mmol) were added to 80mL1,2-dichloroethane for dissolution; then paraformaldehyde (0.75 g, 25mmol), boron trifluoride diethyl ether (3.2mL, 25mmol) were added to the solution, and the reaction was stirred at room temperature for 8h. After the reaction, pour the solution into methanol to precipitate precipitates, filter, dissolve the precipitates with chloroform, extract with 30ml of distilled water x 3 times, dry, and separate the organic phase with column chromatography (petroleum ether/ethyl acetate=50:1v/v) to obtain White solid DCP5-12 (0.95 g), 18% yield.
Step 2. Recognition of Surfactant Cetylpyridinium Chloride Covid by Copolymerizing Pillar 5 Aromatics (DCP5-16)
Make DCP5-16 into 2×10-3mol/L main body solution with CHCl3; respectively take 0.5mL main body solution in 7 colorimetric tubes. Prepare 0.5 mL of different surfactants (cetylpyridinium chloride, cetylpyridinium bromide, cetyltrimethylamine bromide, trimethylamine ethanol solution, triethanolamine, dodecylbenzenesulfonic acid Sodium) in chloroform (concentration: 0.01 mol/L) was used as the guest solution. Add the above-mentioned guest solution to the 7 colorimetric tubes, if the fluorescence of the main solution is quenched, it means that the added guest solution is cetylpyridinium chloride; if the fluorescence of the main solution does not change, it means that the guest solution is not chlorinated cetylpyridine.