The method for colorimetric discovery of Cetylpyridinium Chloride in copolymerized column 5 aromatics in CHCl3 system is as complies with:
Action 1. Synthesis of copolymerized column 5 aromatic hydrocarbon (compound DCP5-16).
1. Synthesis of intermediates: Hydroquinone (4.4 g, 40mmol), salt hydroxide (10g, 4mmol), potassium iodide (4g, 24mmol), hexadecane bromide (26.8 g, 88mmol) and also ethanol (300.0 mL) was included in a 500 mL round-bottomed flask, and also the response was stirred for 3 days (72 h). The solid precipitated as well as the intermediate was gotten. 1HNMR (400MHz, CDCl3, 298K) δ (ppm): 6.82 (d, 4H), 3.96 (t, 4H), 1.75 (t, 4H), 1.43 (t, 4H), 1.26 (t, 48H), 0.86 (s,6 H). The yield is 80%.
2. Synthesis of copolymerized column 5 fragrant hydrocarbons: Add the intermediate (2.79 g, 5mmol) and paraphenylene dimethyl ether (2.76 g, 20mmol) into 80mL of 1,2-dichloroethane to liquify; after that include paraformaldehyde (0.75 g, 25 mmol) and boron trifluoride ether (3.2 mL, 25 mmol) were added to the remedy, and also the reaction was stirred at area temperature level for 8 h. After the reaction, put the option right into methanol to precipitate, filter, liquify the precipitate with chloroform, extract with 30 ml of pure water x 3 times, dry, and separate the natural stage with column chromatography (oil ether/ethyl acetate = 50:1 v/v) to obtain White solid DCP5-12 (0.95 g), return 18%.
Action 2. Copolymerized pillar 5 aromatic hydrocarbon (DCP5-16) recognizes surfactant cetylpyridinium chloride.
Prepare DCP5-16 with CHCl3 to make a 2 × 10-3 mol/L main solution; take 0.5 mL of the major option right into 7 colorimetric tubes. Prepare 0.5 mL of various surfactants (cetylpyridinium chloride, cetylpyridinium bromide, cetyltrimethylamine bromide, trimethylamine ethanol option, triethanolamine, dodecylbenzene sulfonic acid). Salt) in chloroform (focus 0.01 mol/L) was made use of as the visitor option. Add the above visitor services to the seven colorimetric tubes respectively. If the fluorescence of the host option is quenched, it suggests that the visitor service is Cetylpyridinium Chloride; if the fluorescence of the host service does not change, it implies that the visitor solution is not cetylpyridinium chloride.
