The percentage of Cellulose Periodontal is determined by subtracting the following portions of sodium chloride and sodium glycolate from 100.
Sodium chloride material Accurately consider about 5g of the example, transfer it to a 250m1 beaker, include 50ml of water and also 5ml of 30% hydrogen peroxide, heat it on a steam bath for 20min, mix periodically until it is totally dissolved. Cool, make use of silver sulfate as well as mercury sulfate-potassium sulfate electrodes, and also keep mixing, include 100 ml of water and also 10 ml of nitric acid, and after that titrate with 0.05 mol/L silver nitrate to the possible end point. Calculate the portion of sodium chloride in the sample as complies with:
( 584.4 Vc)/( 100-6) ω where V and also c are the volume (m1) and also concentration (mol/L) of silver nitrate consumed, respectively; 6 is the determined loss on drying out; ω is the mass of the sample (g); 584.4 is the molecular weight of sodium chloride.
Salt glycolate material Precisely consider regarding 500mg of the sample, transfer it into a 100ml beaker, wet it with 5ml of antarctic acetic acid and after that 5ml of water, and then stir it with a glass pole till it comes to be a solution (generally it takes around 15min). With stirring, 50 ml of acetone was slowly included, after that 1 g of sodium chloride was included, and also the blend was stirred for numerous mins to precipitate all salt carboxymethylcellulose. Filter through a soft coarse-pored filter paper that has actually been moistened with a percentage of acetone, gather the filtrate in a 100ml volumetric flask, transfer the filter residue to the filter paper with an additional 30ml of acetone and rinse the filter residue, then water down with acetone, and mix well after continuous volume.
Prepare the conventional solution as follows: Properly weigh 100 mg of glycolic acid over night in a desiccator at space temperature, transfer it to a 100-ml volumetric flask, dissolve it in water, make up to quantity and also blend well. The remedy should be utilized within 1 month. Transfer the solution 1.0., 2.0, 3.0 and 4.0 ml right into four 100ml volumetric flasks, respectively, include water to concerning 5ml, then include 5ml of glacial acetic acid, weaken with acetone as well as make up to volume.
Take 2.0 ml of the abovementioned example option and 2.0 ml of each common solution, specifically, transfer them right into five 25ml volumetric flasks, and prepare an empty container containing 2.0 ml of acetone remedy with 5% antarctic acetic acid and water. Each volumetric flask was left uncovered on a boiling water bathroom for 20 min to get rid of acetone, removed, and also cooled down. Include 5.0 ml of 2,7-dihydroxynaphthalene examination option (TS-85) to each container, include 15ml after vigorous mixing, and after that blend intensely. Take a little piece of light weight aluminum foil cover. Place the volumetric flask up and down in a boiling water bathroom for 20min, after that take it out, cool it, make up to quantity with sulfuric acid and also mix well.
Utilize a suitable spectrophotometer, take the empty fluid as a comparison, gauge the absorbance of each fluid at 540nm, draw a typical curve according to the absorbance of the common solution, and after that compute the top quality of glycolic acid in the sample according to the typical contour and also the absorbance of the example (mg), and after that discover the