The extraction approaches of Cardamonin currently consist of ethanol extraction, ether extraction, acetone extraction, chloroform removal, and so on, using silica gel column chromatography. The cardamomine material obtained by these extraction as well as splitting up techniques is reduced, the yield is reduced, and the removal time is long. They are normally made use of as a small-scale preparation approach in chemical make-up lab. Shi Daohua et al. made use of 95% ethanol to soak for 3 days, refluxed with 8 times the amount of ethanol two times, 2 hours each time, recuperated the ethanol under lowered stress to get 600ml of concentrated liquid, and made the concentrated liquid right into a liquid suspension with ethyl acetate as well as n-butanol. Extract in turn, recuperate the solvent under reduced pressure of the essence, and get 340g of extract after concentration. Dissolve the remove in an ideal quantity of acetone, mix the example, and also wet-pack the column with silica gel equivalent to 5 times the quantity of solid, as well as elute with petroleum ether-ethyl acetate system., collect each eluted fraction, spot it on the same thin layer plate as the referral substance, create with toluene-ethyl acetate-methanol (15:4:1) and also chloroform-methanol (20:1) respectively, and make use of UV light (wavelength 365nm), integrate the portions with dark places at corresponding positions of the referral compound, as well as concentrate under typical pressure to get 13g of combined crystals. The mixed crystals were travelled through a silica gel column, and also the eluent was petroleum ether-ethyl acetate-acetone system. After concentration, 5 g of crystals were acquired. Take 100g of the gel and swell it with methanol overnight and also put it right into a column. After the crystals are liquified in methanol, take 20ml and also placed it on the column. Gather each section of 3ml. Examine by thin layer (TLC) and also contrast it with a standard. Integrate the exact same fractions and focus to obtain an orange-yellow needle. 4.2 g of crystals were acquired. Although this method is considerably enhanced than the acetone removal technique, with an extraction price of 0.084%, the extraction price is still very reduced, the extraction time is long, as well as the natural solvent extraction price is reduced as well as the loss is large.
CN201010235806.0 provides a technique for extracting Cardamonin with high extraction price, brief cycle and also high pureness.
Technical solution: In order to accomplish the above function, the invention supplies an approach for drawing out cardamomine from cardamom, which especially consists of the following actions: a. Removal step: take a suitable amount of cardamom seeds, crush them to 20-60 mesh, and also cool them. By seepage, cozy soaking or reflux removal method, cardamom seed powder is drawn out with solvent 1-3 times, the essences are integrated, and focused under minimized pressure to get an essence; b. Organic solvent removal action: extract the extract with oil ether up until the essence is obtained Nearly colorless, discard the petroleum ether essence and also maintain the staying extract; c. Splitting up and filtration action: Usage column chromatography to divide the continuing to be remove with water, methanol, ethanol, ethyl acetate, acetone, dichloromethane or their mixes The combined solution is eluted, kept track of by high performance liquid chromatography, as well as the effluent having Cardamonin is collected. The effluent is concentrated and then crystallized with an organic solvent to get high-purity cardamomine. Compared with the existing technology, the cardamomine extraction method provided by this technique has the adhering to benefits: (1) The approach course offered by the present development is reasonably developed, and the method is simple and also easy to run; (2) The method offered by the existing innovation has a brief cycle and high extraction price. High, item purity is high; (3) The solvent and column chromatography filler utilized in the approach provided by the creation can be recuperated and also reused, which can reduce prices as well as is a low-cost, reusing procedure.
