Record 1
The method for extracting betaine from beet molasses includes the adhering to actions:
( 1) Procedure beetroot molasses: betaine web content is 7% and overall sugar content is 46%. Placement it in a water bathroom, include distilled water to deteriorate it at a proportion of 1:1.8, and stir at 55 ° C.( 2) Pass the thinned down feed fluid with a ceramic membrane layer with a pore size of 80nm. Throughout the microfiltration process, control the temperature degree to 65 ° C, the feed pressure to 0.2-0.25 MPa, the discharge stress to 0.15-0.20 MPa, and the membrane layer change to 60LMH. The amount of water consisted of is 32% (v/v) of the overall amount of the feed, and the penetrate is gathered after purification. Betaine return is 99%;.
( 3) Utilize a nanofiltration membrane (rolled membrane layer) with a molecular weight of 250D for treatment. The inlet pressure of the nanofiltration membrane layer is 2 to 2.5 Mpa, the electric outlet stress is 1.5 to 2.0 Mpa, the modification is 18LMH, and the amount of dialysis water included is 42% of the overall volume of the microfiltrate feed. % (v/v), the nanofiltration fluid was built up, and the betaine return reached 97.5%.
( 4) Pass the penetrate right into the 7th chromatographic column of the chromatography system, elution water gets in from the 1st chromatographic column, product fluid eruptions from the third chromatographic column, wastewater drains from the 9th chromatographic column, filter During the process, the temperature degree of each chromatographic column was managed to 75 ° C, and the betaine internet content of the item fluid was 96%, and the chromatographic betaine return was 93%;.
( 5) Concentrate the item fluid to a solid internet material of 72%, after that reduced the temperature level to 18 ° C for development. After the formation is finished, centrifuge for solid-liquid dividing.
( 6) Dry the crystals at 60 ° C to get a completed product of betaine. The pureness of the ended up product is 98.6%, and the total betaine yield is 80.8%.
Record 2
A strategy for drawing out Betaine Anhydrous from wolfberry fallen leaves, consisting of the complying with actions:.
( 1) After by hand choosing and removing contaminants from the wolfberry leaves, entirely dry them in the color inside to get dried wolfberry fallen leave examples.
( 2) Area 4kg of dried wolfberry dropped leave example right into a crusher for crushing, and afterwards pass it with a 40-mesh screen to obtain wolfberry leaf powder.
( 3) Mix wolfberry fallen leave powder and water at a material-to-liquid mass quantity ratio (kg/L) of 1:10, and essence the remove at 10 ° C for 10 hours.
( 4) Use a centrifuge to centrifuge the extract at a centrifugal price of 4000r/min for 5 mins to get the supernatant and filter residue. Build up the supernatant and dispose of the filter down payment.
( 5) After the filtrate is adsorbed by the medium-polar macroporous adsorption material, it is first eluted with deionized water up till it is sugar-free, and after that the wolfberry adsorbed on the macroporous material is eluted with a methanol-water-acetic acid choice of 2 times the bed amount. dropped leave betaine to get an eluate having wolfberry dropped leave betaine.
Amongst them: the quantity ratio (ml/ml/ml) in between methanol, water and acetic acid in the methanol-water-acetic acid alternative is 6:2:1.
( 6) Focus the eluate having Lycium barbarum leaf betaine under lowered anxiety at a vacuum cleaner level of 0.03 MPa and a temperature degree of 30 ° C to obtain a Lycium barbarum leaf betaine focused fluid; the Lycium barbarum dropped leave betaine focused fluid is acquired Dry to consistent weight at 40 ° C to get 1.15 kg of tan wolfberry fallen leave betaine powder.
After high-performance fluid chromatography exploration, the betaine web content in the wolfberry dropped leave betaine powder was 11.2%.
Record 3
The prep work technique of Betaine Anhydrous is eliminated from wolfberry origins. The roots of wolfberry plants are used as basic materials, and betaine is prepared by a mix of removal, adsorption, elution, focus and other technologies. It has the qualities of affordable and high elimination efficiency, particularly Utilize the following setup strategy: consist of the complying with activities:.
1) Clean the origins of wild wolfberry plants, reveal them to the sun for 1 to 2 hours, and later on completely dry them in the color under natural ventilation problems to obtain a dried wolfberry origin example with a moisture web content of 0.1 to 0.2%;.
2) Cut the dried out wolfberry origin sample right into items and squash it, pass it with a filter of 80 to 280 mesh to acquire wolfberry root powder;.
3) Utilize removal, adsorption, elution, and focus techniques to prepare wolfberry origin betaine from wolfberry leaf powder to obtain wolfberry beginning betaine concentrate;.
4) Vacuum-dry the wolfberry origin betaine concentrate to obtain betaine powder.
Record 4
A method for dividing and detoxifying wolfberry betaine, consisting of the complying with actions:.
( 1) Dry the wolfberry in the color at space temperature degree for a week up until the wolfberry is squashed right into industries to acquire a dried example of the wolfberry.
( 2) Cut the dried out wolfberry sample right into items to get the crushed wolfberry instance.
( 3) Mix the crushed wolfberry examples with water at a material-to-liquid mass amount ratio of 1:5 g/mL, and get rid of the eliminate at 10 ° C for 0.5 hours.
( 4) Centrifuge the significance at 8000r/min for 15 minutes to obtain supernatant I and filter deposit I;.
( 5) After concentrating the supernatant I under vacuum and lowered pressure at 45 ° C, include 6 times the quantity of straight-out ethanol to the concentrated alternative to blend and get rid of sugar, to acquire a combined solution;.
( 6) Centrifuge the combined treatment at 8000r/min for 15 minutes to obtain supernatant II and filter residue II.
( 7) Concentrate the supernatant II under vacuum cleaner and stress at 45 ° C to acquire an essence. Include a suitable quantity of double-distilled water to liquify the extract to get an example treatment.
( 8) After the example alternative is adsorbed by the 732 type cation exchange material, initial clean it with adequate deionized water up till the eluent turns light yellow or anemic, and after that usage 2 times the bed quantity of ammonia water to elute and adsorb the 732 kind cation exchange material. Betaine on the cation exchange product was used to obtain an eluent consisting of wolfberry betaine, where the volume emphasis of ammonia was 5%.
( 9) After adsorbing the wolfberry betaine-containing eluate with 717-type anion exchange material, use 4 times the bed quantity of deionized water to elute, and collect the effluent. After the effluent is concentrated under hoover and minimized tension, a concentrated fluid of wolfberry betaine is obtained.
( 10) Lyophilize the wolfberry betaine concentrate to obtain crude wolfberry betaine.
( 11) Clean the unrefined wolfberry betaine two times with ethanol, and centrifuge at 8000r/min for 15 minutes to get supernatant III and filter residue. Location the supernatant III in an instance container and concentrate to dry skin under lowered pressure., get wolfberry betaine.
After high-performance fluid chromatography detection, the betaine product of the wolfberry fruit was 81.54%.
