Zhishang Chemical

Welcome to Zhishang Chemical      +86-176 5311 3209     Simon@sdzschem.com

Zhishang Chemical

Beta Leucine CAS 61-90-5

Molecular Formula: C6H13NO2

Formula Weight: 131.17

ZSpharmac: Beta Leucine Supplier

Product Name: Beta Leucine
CAS No: 61-90-5
Purity: 99%

Navigation Bar

Basic Info

Product Name:Beta Leucine
Other Names:L-Leucine
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:White to Off-White Powder
EINECS No.:200-522-0
Provide:Beta Leucine MSDS;
Beta Leucine COA

What is Beta Leucine?

Beta Leucine (L-leucine), likewise called leucine, is α-amino-γ-methylpentanoic acid, α-aminoisocaproic acid, and also its molecular formula is C6H13O2N. Proust was first isolated from cheese in 1819, as well as later Braconnot was taken shape from the acid hydrolyzate of muscular tissue as well as wool, as well as called leucine.

Beta Leucine Property

Melting point 

>300 °C (lit.)


15.4 º (c=4, 6N HCl)

Boiling point 

122-134 °C(Press: 2-3 Torr)


1,293 g/cm3

vapor pressure 

<1 hPa (20 °C)

refractive index 

1.4630 (estimate)



storage temp. 



1 M HCl: 50 mg/mL




2.328(at 25℃)


White to Off-white


5.5-6.5 (20g/l, H2O, 20℃)

optical activity

[α]20/D +15.5°, c = 2 in 6 M HCl

Water Solubility 

22.4 g/L (20 C)


Beta Leucine Uses

  1. Beta Leucine is used in dietary supplements; seasoning as well as flavoring agents. Prep work of amino acid infusion and also thorough amino acid prep work, hypoglycemic agents and also plant development marketers. According to my country’s GB 2760-96 policies, it can be used as a seasoning.
  2. Beta Leucine is made use of in biochemical research study, in medicine for the treatment as well as medical diagnosis of idiopathic hyperglycemia in children, and also in the treatment of anemia, poisoning, muscle atrophy, sequelae of poliomyelitis, neuritis and mental disease.
  3. Beta Leucine is made use of as amino acid infusion and extensive amino acid preparation. It is made use of for the medical diagnosis and therapy of idiopathic hyperglycemia in little ones, as well as conditions of sugar metabolism, liver disease with reduced bile secretion, anemia, poisoning, muscle atrophy, sequelae of poliomyelitis, neuritis as well as mental disorder, etc. Diabetes mellitus, cerebrovascular sclerosis as well as kidney disease patients with proteinuria as well as hematuria ought to not be made use of. Patients with stomach and also duodenal ulcers should not take it.
  4. Beta Leucine is a vital amino acid, the adult man requires 2.2 g/d, which is required for the typical growth of babies and also the upkeep of normal nitrogen balance for adults. This product is made use of as a nutritional supplement to prepare amino acid mixture as well as extensive amino acid prep work, hypoglycemic agents, and plant growth promoters. According to the arrangements of my country’s GB2760-86, this item can be made use of as a flavor.

Fermentative Production Method of Beta Leucine

A method for producing Beta Leucine by fermentation

(1) Activation culture: Inoculate the Beta Leucine producing bacteria (i.e. Corynebacterium glutamicum ACCC16522, purchased from Shanghai Fuxiang Biotechnology Co., Ltd.) onto the activation medium, and activate the culture in an incubator at 32°C for 12 hours to obtain the activated bacteria .

Activation medium: anhydrous glucose 1g/L, peptone 10g/L, beef extract 10g/L, yeast extract 5g/L, NaCl 2.5g/L, agar powder 20g/L, pH=7.0-7.2.

(2) Seed culture: Take a ring of well-growing activated bacteria and inoculate it into a shake flask containing seed medium for seed culture. The liquid volume is 200mL/500mL. The conditions for seed culture are 32°C, 150r/min shaker culture After 20 hours, the concentration of bacteria in the fermentation broth was measured with a spectrophotometer. If the OD620 value of the bacteria reached 1.2, it could be used as a qualified seed solution to obtain a seed solution.

Seed medium: anhydrous glucose 20g/L, corn steep liquor powder 25g/L, ammonium sulfate 2g/L, KH2PO4·3H2O 1.5g/L, vitamin B1 300μg/L, biotin 200μg/L, pH=7.0-7.2.

(3) Fermentation culture: according to the inoculum amount of 10%, get the seed liquid that step (2) obtains, inoculate in the 10L fermentor that contains fermentation medium, the filling amount is 6L/10L, and culture temperature is 30 ℃-35 ℃, the stirring speed is 600rpm/min, feed medium is added during the cultivation process, the ventilation volume is 80-100L/h in the first 12h, and the ventilation volume after 12h is 100-120L/h, and the fermentation process is controlled by automatic flow of ammonia water Medium pH=6.6±0.05, cultured for 30-32 hours to obtain fermentation broth. The end time of fermentation was judged by the fact that acid production no longer increased. Before and after fermentation, the content of Beta Leucine was determined by high performance liquid chromatography; the content of residual sugar in the fermentation liquid was detected by a biosensor analyzer.

Fermentation medium: sodium malate 2.0-4.0g/L, anhydrous glucose 140-160g/L, corn steep liquor dry powder 35g/L, ammonium sulfate 20g/L, KH2PO4 3H2O 1.0g/L, MgSO4 7H2O 0.4g/L L, MnSO4·H2O 0.1g/L, vitamin B1 300μg/L, biotin 50μg/L, CaCO3 30g/L, defoamer 0.5%, pH=7.0-7.2.

Feed medium: sodium malate 1.0g/L, inositol 1.0-2.0g/L, corn steep liquor powder 12g/L, ammonium sulfate 25g/L, KH2PO4 3H2O 2.0g/L, MgSO4 7H2O 0.6g/L , MnSO4·H2O 0.3g/L, vitamin B1 400μg/L, biotin 60μg/L, CaCO3 20g/L, defoamer 1.0%, pH=7.0-7.2.

(4) Extraction of L-leucine: take the fermentation broth after fermentation, centrifuge at 8000r/min for 10min, collect the supernatant, extract and purify the supernatant through an ion exchange column, load the sample at a flow rate of 0.5BV/h, and load The sample volume is 260mL of fermentation broth, the eluent used is 3.0mol/L ammonia water, the elution flow rate is 0.1-1.0BV/h, the effluent is collected, and the Beta Leucine in the fermentation broth and effluent is determined by high performance liquid chromatography The content of the calculated yield.

Production Method of Beta Leucine

About Products:
If you need Beta Leucine, the cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced Beta Leucine manufacturer and supplier, ZSpharmac will provide Beta Leucine raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

Get Beta Leucine Quotation