Beta Karotenas CAS 7235-40-7

1. Extraction from plants can be extracted from carrots, peppers or silkworm sand with organic solvents such as petroleum ether. When extracting lutein, the non-soap of the gasoline layer is washed once with an equal amount of 1% sodium chloride solution to remove soluble lipid compounds, and the lower aqueous phase is removed by standing and stratified. The gasoline layer was distilled under reduced pressure to recover gasoline to obtain a yellow waxy residue, which was dissolved in petroleum ether and quickly filtered. The filtrate was passed through an activated alumina chromatography column with petroleum ether:acetone=8:
The mixture of

2. (volume ratio) is eluted, and the solvent is recovered by distillation under reduced pressure to obtain an oily crude β-carotene. Then use anthracene-ethanol for recrystallization to obtain finished crystals. This method is limited by resources and cannot be mass-produced. (2) Chemical synthesis method The chemical synthesis method is the Grignard reaction method of the Swedish Roche company and the Witting reaction synthesis method of the German Base company. The Grignard reaction synthesis method uses β-ionone as raw material to obtain β-C14, β-C16, β-C19 aldehyde successively, and then two molecules of β-C19 aldehyde are condensed into β-C40 diphenol by Grignard, and then dehydrated, Hydrogenated. The overall yield was 21% based on β-ionone. The Wittig reaction synthesis method also uses β-ionone as the raw material to synthesize β-carotene through vinyl-β-violet, C15+C10+C15, and the yield is 25% in terms of C15 enol. See the literature for details on the reaction formula. Although the synthetic method has a long route, it is more economical, and has good nutritional value and color stability.

3. Extraction from salina salina is a marine plankton that grows in salt pans. my country has a long coastline, rich potential resources and good development prospects. The following two technological routes are proposed in the national “Seventh Five-Year” public relations project. (1) Petroleum ether extraction process. The wet salina powder was extracted with No. 6 solvent oil under stirring at 50°C for about 20 minutes, and the filter residue was filtered and extracted twice. The filtrate is concentrated by thin-film evaporation, crystallized, filtered, and vacuum-dried to obtain the finished product, and the extraction rate is about 80%. (2) Vegetable oil extraction process. Extract with 2 times the vegetable oil of dry salina powder at 75°C with stirring for 30-60min. The oil solution of beta-carotene is obtained by centrifugation by a three-legged centrifuge, and then by centrifugation by a disc centrifuge.

4. Preparation of fermentation method The red yeast cells containing beta-carotene were cultured by fermentation method. The wort with a relative density of 1.06-1.07 was used as the medium, 2%-3% of red yeast was inoculated after high temperature sterilization, and the culture was aerated for 48 hours under the conditions of 26 ° C and PH5-6, and the fermentation broth was separated by high-speed centrifugation for 15 minutes. , washed with distilled water, and dried at 50 °C to obtain dry yeast cells. The stem cells were first treated with 2-3mol/L hydrochloric acid, and then boiled in boiling water for 2-3min. Cell debris was obtained by high-speed centrifugation and washing twice with water. The stem cell fragments were soaked in acetone, and the cell fragments were removed by high-speed centrifugation to obtain a beta-carotene extract. Concentrated under reduced pressure by rotary thin film evaporator, and extracted with chloroform to obtain β-carotene concentrate. Finally, it is concentrated under reduced pressure and spray-dried to obtain the finished product.