1. When benzethonium chloride and PHMB are used alone, the sterilization rate is lower than that of mixed use, that is, the two fungicides have a synergistic sterilization effect. And when the mass fraction of benzethonium chloride was about 50%, the bactericidal rate reached the highest value, the bactericidal rate of the mixed bactericide against Escherichia coli was close to 80%, and the bactericidal rate against Staphylococcus aureus was close to 90%. That is, when the two fungicides are configured in a ratio of 1:1, the best synergistic effect can be exerted. The two fungicides showed a synergistic bactericidal effect. The possible reason is that both of them are cationic surfactants, but the relative molecular weights of the two fungicides are quite different. The relative molecular mass of benzethonium chloride is small, and the relative molecular mass of polyhexamethylene biguanide is high, so the sterilization mechanism of the two may be different. Therefore, when they are used in combination, the bacteria will be destroyed according to their respective sterilization mechanisms, and the overall mortality of the bacteria will increase.
2. The above explores the synergistic effect of the total mass fraction of mixed fungicides of 0.0005%. When the ratio of benzethonium chloride and PHMB is 1:1, the bactericidal rate of Escherichia coli and Staphylococcus aureus can reach 80% respectively. , 90%. In the actual production of sanitary products, the sterilization standard is 90%. Therefore, it is necessary to explore the synergistic effect of the total mass fraction of mixed fungicides higher than 0.0005%.
3. Benzethonium chloride and PHMB are configured in a ratio of 1:1 to achieve the best synergistic effect, then the ratio of the two in the mixed fungicide is determined to be 1:1, and the total mass fraction of the tested mixed fungicide is 0.001% and 0.005%. %.
When benzethonium chloride and PHMB are used alone, the bactericidal rates of the two against Escherichia coli and Staphylococcus aureus are both close to 100% when the mass fraction is 0.02%; the other two have synergistic bactericidal effect, and when the two are 1: 1: The best sterilization effect can be achieved when the proportion of 1 is used in a mixed configuration. Under the optimal ratio of the two (1:1), the sterilization rate of the above two bacteria is close to 100% when the total mass fraction of the mixed bactericide is 0.005%.