1. Cold soak extraction method: Because Aucubin is quickly soluble in methanol, 30% methanol option is utilized for extraction. When left at space temperature, the shade of the remedy adjustments. The greater the area temperature level, the much faster the adjustment. Nevertheless, when the example is put in the fridge overnight throughout extraction, the color of the example remedy does not change, and there is no change when it is put again. Therefore, chilly removal with 30% methanol remedy guarantees that the sample solution is steady. This technique is basic and affordable, but takes a long time to remove.
2. Alcohol removal of lead salt rainfall technique: Remove with ethanol, concentrate the remove to prepare an extract, add water to dissolve, then add lead salt to precipitate, filter the precipitate, pass the filtrate right into H2S to remove lead, and concentrate under decreased pressure. Liquify the deposit with acetone, focus the acetone option to acquire unrefined aucubin, and lastly recrystallize to obtain the pure item. Due to the fact that the lead rainfall method has certain selectivity for aucubin, this approach can obtain reasonably pure Aucubin. However, because of making use of poisonous heavy metal lead, it might trigger certain pollution to aucubin items.
3. Ultrasonic extraction approach: Ultrasonic wave is a consistent round mechanical wave that creates solid shock waves and micro jets inside the fluid through an one-of-a-kind kind of activity – cavitation, which can break the cell wall surface and permit intracellular parts to penetrate right into the solution., thus accomplishing the purpose of splitting up. The ultrasonic removal method is straightforward and quick to run, has the advantages of conserving time, and does not require home heating. It can be made use of to remove some substances that are conveniently ruined and decayed by warmth. It has low overall price, much less air pollution, and the essence is very easy to divide. It can attain better results than standard removal techniques. More excellent outcomes. On top of that, this approach is easy and suitable for commercial production, but it is currently restricted to lab range.
4. Macroporous resin adsorption method: Macroporous adsorption resin is a polymer adsorbent that does not contain exchange groups and has a macroporous structure. It is also a lipophilic substance. It has the advantages of good selectivity, high mechanical stamina, convenient regeneration handling, rapid adsorption speed, simple procedure, steady product quality, and reduced production price, so it is specifically suitable for dividing and cleansing water-soluble substances. However, this method takes a very long time and is tough to clean up the adsorption material. The adsorption materials presently utilized to cleanse Aucubin primarily include S-8 material and D4020 material. Among them, S-8 resin has great selectivity and huge adsorption capability. It can be entirely eluted with 60% ethanol aqueous option.