1. Cold saturating extraction approach: Given that aucubin is quickly soluble in methanol, it is extracted with 30% methanol remedy. When put at space temperature, the color of the remedy adjustments, and the higher the room temperature, the faster the adjustment. However, when the example is put in the fridge over night during removal, the shade of the sample option remains unmodified, and there is no change when it is placed once again. Therefore, the example service was steady when extracted by chilly immersion in 30% methanol solution. The process of this approach is basic and affordable, however the removal time is much longer.
2. Alcohol extraction of lead salt precipitation technique: essence with ethanol, concentrate the essence to prepare extract, include water to liquify, after that add lead salt to precipitate, filter the precipitate, pass the filtrate right into H2S to remove lead, and also concentrate under reduced pressure. The deposit was dissolved in acetone, and the acetone remedy was focused to acquire the crude aucubin, which was ultimately recrystallized to acquire the pure item. Due to the fact that the lead precipitation method has a specific selectivity to aucubin, this method can acquire fairly pure aucubin, however as a result of making use of hazardous heavy metal lead, it might create specific contamination to aucubin products.
3. Ultrasonic extraction approach: Ultrasonic wave is a consistent spherical mechanical wave, which generates strong shock waves as well as micro jets inside the fluid with an unique type of activity – cavitation, which can break the cell wall and also permit intracellular components to pass through right into the service, so regarding accomplish the function of splitting up. Ultrasonic extraction approach is simple and quick to run, has the advantages of saving time and also no demand for home heating, as well as can be utilized to remove some substances that are conveniently damaged and decayed by heating, as well as has reduced overall price, much less contamination, and the extract is very easy to divide, which can reach a greater level than conventional extraction techniques. better outcomes. On top of that, the approach is straightforward as well as ideal for commercial manufacturing, yet it is just restricted to lab scale.
4. Macroporous material adsorption technique: macroporous adsorption material is a polymer adsorbent without exchange groups as well as has a macroporous framework, as well as it is also a lipophilic substance. It has the advantages of great selectivity, high mechanical stamina, practical regrowth treatment, rapid adsorption speed, simple procedure, stable product high quality and also reduced manufacturing cost, so it is especially ideal for the separation and also filtration of water-soluble compounds. Yet this method takes a long time and it is challenging to cleanse the adsorption resin. Currently, the adsorption materials utilized to cleanse aucubin generally include S-8 material and D4020 material, among which S-8 resin has excellent selectivity and also big adsorption ability, and also it can be totally cleansed by 60% ethanol aqueous option. elute.