Weigh 500g of pulverized Centella asiatica powder, soak in 2500ml mass concentration of 75% ethanol aqueous solution, ultrasonically extract 4 times at 50°C, each time for 1 hour, filter, combine the filtrates and concentrate under reduced pressure to obtain 20.5g of extract; Dissolve the extract in 200ml of water, extract 3 times with an equal volume of petroleum ether in the aqueous extract solution, extract 4 times with an equal volume of ethyl acetate in the lower phase aqueous solution, and collect the lower phase aqueous solution. Use the same volume of n-butanol and ethyl acetate as the volume ratio of the lower phase aqueous solution to extract 3 times, combine the upper phase extracts, decolorize the extracts with activated carbon, and concentrate the decolorized extracts under reduced pressure to obtain a product. Madecassoside 7.3g; add 10 times the amount of methanol to dissolve asiaticoside, filter, concentrate the filtrate, and recrystallize with 90% ethanol aqueous solution at room temperature to obtain 3.3g of high-purity asiaticoside , the purity is 90%, and the yield is 0.66%.
1) Crush Centella asiatica and add water, the amount of water used is 5 times the weight of Centella asiatica, reflux extraction once at 40°C, each time for 0.5h, filter the extract to obtain a crude extract;
2) Put the crude extract on the XAD-10 macroporous resin column, the crude extract is loaded on the column at a speed of 3BV/h, and eluted with an acid solution with a pH value of 2, and the elution rate of the acid solution is 1BV/h, and the combined flow liquid and pickling liquid;
3) Adjust the pH value of the combined solution to neutral, filter, put the filtrate on the XAD-3 macroporous resin column again, the column speed of the filtrate is 0.5BV/h, elute with ethanol with a volume concentration of 30%, wash with ethanol The detachment rate is 2BV/h, followed by thin-layer chromatography detection, collecting the ethanol eluate containing the target component, concentrating, and drying to obtain asiaticoside.
By HLPC analysis, the recovery rate of asiaticoside was over 86.24%, and the purity was as high as 95.44%.
Centella asiatica is crushed, passed through a 80-mesh sieve, and 1kg of powder is taken, and the compound enzyme equivalent to 1% of centella asiatica powder (the weight ratio of cellulase, hemicellulase and pectinase is 0.5:0.3:0.2) is sprayed On it, wrapped with film, stacked in a dark room with a temperature of 35°C, fermented for 24 hours, added 8 times of 50% ethanol to the fermented raw materials, placed in a dual-frequency ultrasonic extractor, the ultrasonic frequencies were 40KHz and 80KHz, and the power was 400W, extract at 60°C for 60min, filter, concentrate, extract with petroleum ether-water, remove the lower layer, put it on an alumina column, elute with 10BV of 50% ethanol, concentrate the eluate, and recrystallize twice from methanol to obtain Centella asiatica Glycoside 10.2g, the purity is 90.5%, and the yield is 1.02%.