Since Chasseaud et al. determined that Asiatic Acid itself has the effect of treating skin wounds in 1971, Centella asiatica extract (mainly containing asiatic acid and asiaticoside) has been used for the treatment of skin wounds and chronic ulcers, and also for the treatment of skin wounds. Deformation of the skin due to tuberculosis and leprosy. Usually the skin is easily directly exposed to ultraviolet rays (UVA) and is the organ most susceptible to UVA. Pre-aging of the skin, known as photoaging, occurs after UVA damage. The UVA-mediated signal transduction pathway ultimately leads to photoaging, and the two most important reasons are the excessive production of oxygen free radicals and the appearance of matrix metalloproteinases (MMPs).
Irradiation of HaCaT keratinocytes with UV light can induce oxidative stress, enhance the expression of MMP-2, and cause changes in UVA-mediated signaling pathways. Asiatic acid can significantly inhibit UV-induced generation of oxygen free radicals and lipid peroxidation. After treatment with 5 μM asiatic acid, the expression level of UVA-induced MMP-2 was decreased, and the activity of MMP-2 protein was decreased. In addition, UVA was able to increase p53 expression in HaCaT cells, which is characteristic of UVA-induced DNA damage and cell death. This process was also significantly inhibited by asiatic acid pretreatment. Therefore, asiatic acid can effectively inhibit skin damage caused by UVA and prevent UVA-induced photoaging. By comparing the effects of asiatic acid, madecassoside and madecassoic acid on wound healing in rats, it was found that Asiatic Acid was the most effective ingredient in promoting wound healing.