Consist of the complying with actions:
S1. Add pure water into the response pot, after that include vitamin C into the reaction kettle, mix and mix until attire.
S2. Maintain the temperature in the activator at 28-32 ° C, and change the pH to 6.05 ± 0.10 with 30% NaOH remedy.
S3. Include β-cyclodextrin right into the reaction pot.
S4. After that include cyclodextrin glycosyltransferase to the response pot as well as distilled water to make the system measured; regulate the temperature in the response pot to 33 ± 4 ° C., avoid light for S10-12h.
S5. After the reaction S1 is finished, add glucoamylase, manage the temperature level at 35 ± 2 ° C, and avoid light for response S10-14h.
S6. After the reaction is completed, discharge the products, wash the reaction pot with pure water, incorporate the reaction service and the kettle cleaning water, evaluate them, as well as centrifuge via a centrifuge, as well as wash the centrifuge with pure water once again.
S7. Ultrafiltration is performed on the acquired filtrate via an ultrafiltration equipment, distilled water is included during the ultrafiltration process, and top cleaning is performed with distilled water, and also the temperature is controlled to be lower than 28 ° C.
S8. Pass the ultrafiltrate through the first resin column, and pass the eluate into the nanofiltration maker.
S9. Wash the initial resin column with pure water, and pass the eluent right into the nanofiltration machine.
S10. Elute the resin with sodium chloride solution, pass the eluent with the first resin column, and elute with pure water up until no item spurts, and also integrate all the eluents.
S11. Pass the consolidated eluent via a nanofiltration equipment, after that clean with pure water, lastly concentrate, and top clean with pure water; transfer the nanofilter to a continual liquid splitting up gadget to separate the sugar, so that the material of the Ascorbyl Glucoside Powder in the splitting up solution is 45%. -50%.
S12. Pass through the second material column, readjust the pH via the cation resin, and afterwards focus with the nanofiltration membrane to eliminate the inorganic salt in the reaction service to obtain the vitamin C glucoside concentrate.
S13. Pass the focused remedy of vitamin C glucoside through the titanium rod filter gadget while it is hot, evaluate the filtrate, and send it for inspection of the content of L-ascorbyl glucoside.
S14. Include the L-ascorbyl glucoside filtrate right into the response kettle, concentrate under decreased pressure, control the temperature ≤ 45 ° C, and vacuum ≥ -0.07 Mpa; concentrate until the web content of L-ascorbyl glucoside in the option is 72% ± 1%.
S15. After the focus is finished, control the temperature level of the pot to 36-40 ° C, include seed crystals, stir, after that allow it represent sluggish crystallization, and slowly cool for 8 hrs. After the temperature level drops to 32 ° C, program the temperature level down to 5-10 ° C, begin stirring, and then 5 Crystallization at -10 ° C. S16. After the condensation is finished, discharge and centrifuge till no fluid decreases drain, then rinse with ice water spray, continue centrifuging until no fluid declines spurt, and afterwards proceed centrifuging for half an hour, and discharge to acquire L-ascorbyl glucoside top notch wet item.
S17. After the centrifugation, the acquired L-ascorbyl glucoside damp product is dried out, the temperature level control is ≤ 30 ° C, and also the vacuum cleaner level is <-0.1 Mpa; after drying, the fine L-ascorbyl glucoside is obtained.
