S1. Add distilled water into the reactor, then add vitamin C into the reactor, mix and stir until uniform.
S2. Keep the temperature in the reaction kettle at 28-32°C, and adjust the pH to 6.05±0.10 with 30% NaOH solution.
S3. Add β-cyclodextrin into the reaction kettle.
S4. Add cyclodextrin glycosyltransferase to the reaction kettle and add distilled water to make the system quantitative; control the temperature in the reaction kettle to 33±4°C, and react in the dark for S10-12h.
S5. After the reaction S1 is completed, add saccharification enzyme, control the temperature to 35±2°C, and react in the dark for S10-14h.
S6. After the reaction is completed, the material is discharged, the reaction kettle is washed with distilled water, the reaction solution and the washing water are combined, weighed, centrifuged by a centrifuge, and the centrifuge is washed with distilled water again.
S7. The obtained filtrate is subjected to ultrafiltration through an ultrafiltration machine, and distilled water is added during the ultrafiltration process, and top-washed with distilled water, and the temperature is controlled to be lower than 28°C.
S8. Pass the ultrafiltrate through the first resin column, and pass the eluent into the nanofiltration machine.
S9. Rinse the first resin column with distilled water, and pass the eluent into the nanofiltration machine.
S10. Elute the resin with sodium chloride solution, pass the eluate through the first resin column, and elute with distilled water until no product flows out, and combine all the eluates.
S11. pass the combined eluent through a nanofiltration machine, then wash with distilled water, finally concentrate, and top-wash with distilled water; transfer the nanofiltrate to a continuous fluid separation device to separate out glucose, so that the L-ascorbic acid glucoside content of the separation solution is 45%-50%.
S12. After passing through the second resin column, adjusting the pH through the cation resin, and then concentrating through a nanofiltration membrane to remove the inorganic salts in the reaction solution to obtain a concentrated solution of vitamin C glucoside.
S13. Pass the vitamin C glucoside concentrate while hot through a titanium rod filter device, weigh the filtrate, and send the L-ascorbyl glucoside content for inspection.
S14. Add the L-Ascorbyl Glucoside Deciem filtrate into the reaction kettle, concentrate under reduced pressure, control temperature ≤ 45°C, and vacuum degree ≥ -0.07Mpa; concentrate until the content of L-Ascorbyl Glucoside in the solution is 72%±1%.
S15. After the concentration is completed, control the temperature of the kettle to 36-40°C, add seed crystals, stir, then stand for slow crystallization, slowly cool down for 8 hours, and after the temperature drops to 32°C, program the temperature to 5-10°C, start stirring, and then 5 -10 ℃ thermal insulation crystallization.
S16. After the crystallization is completed, the material is discharged and centrifuged, centrifuged until no droplets flow out, and then rinsed with ice water spray, and the centrifugation is continued. After centrifugation until no droplets flow out, the centrifugation is continued for 30 min, and the L-ascorbyl glucoside fine wet product is discharged.
S17. The L-Ascorbyl Glucoside Deciem wet product obtained after the centrifugation is dried, the temperature is controlled to be less than or equal to 30°C, and the vacuum degree is less than -0.1 Mpa; after the drying, the L-Ascorbyl Glucoside Deciem is obtained.
