Tiny recognition
Cross section of the main root: The cork layer is made up of numerous rows of cork cells, yellowish brownish. The cortex is narrow, and some cells include calcium oxalate collections. There are commonly splits on the outside of the phloem, and resin canals are spread, having yellow-brown secretions. The cambium layer is noticeable. Xylem vessels are scattered singly or in several collections, set up intermittently in a radial pattern. Parenchyma cells are full of starch granules.
Physical and chemical identification
Take 0.5 g of the powder, include a proper quantity of water to dampen it, include 2ml of water-saturated n-butanol, and carry out ultrasonic therapy for 20 minutes. Vaporize the supernatant to dry skin, and liquify the residue with 1ml of methanol to serve as the examination remedy. In addition, take 0.5 g each of American ginseng reference medical product and ginseng recommendation medicinal product, and prepare American ginseng recommendation medicinal material service and ginseng recommendation medical product referral remedy similarly. Additionally, ginsenoside-Rb1, -Re, and -Rg1 referral requirements methanol options (1 mg/ml) were prepared respectively. Take 5 μl of each of the above six services, position them on the same silica gel G thin layer plate, and make use of chloroform-ethyl acetate-methanol-water (15:40:22:10) [or chloroform-methanol-water (65:35: 10)] The lower layer service left at 5-10 ° C for 12 hours is established, splashed with 10% sulfuric acid ethanol service, baked at 105 ° C until the spots are clear, and examined under sunlight and ultraviolet light (365nm). The chromatogram of the examination product revealed places of the exact same color at the matching settings as the chromatogram of the American ginseng recommendation medicinal product and the reference material chromatogram, but they were various from the chromatogram of the ginseng reference medicinal material.
UV range recognition
Take a suitable quantity of sample powder, make use of a Soxhlet extractor to reflux with diethyl ether in a water bath for 2 hours, and essence the residue with methanol reflux numerous times. Recuperate the methanol to dryness under lowered pressure, include pure water to dissolve, and remove 5 times with water-saturated n-butanol. The n-butanol remove was recuperated under decreased stress till it was dry, and afterwards liquified in methanol to develop a methanol remedy including 3 mg of the initial medical material per 1 ml. Take 1.5 ml of example remedy, add 0.6 ml of focused sulfuric acid, shake well, warmth on an 80 ° C water bathroom for 60 mins, include ethanol to 10 ml, and determine its UV spectrum. Outcomes American ginseng had a maximum absorption height at a wavelength of 275 ± 1nm.
