Allura Red Color CAS 25956-17-6

(1) Prep work of polysulfosalicylic acid-terpyridine ruthenium-modified glassy carbon electrode
Location the glassy carbon electrode in a liquid option including 1.0 × 10-2mol/ L sulfosalicylic acid and 1.0 × 10-4mol/ L ruthenium terpyridine, as well as perform electropolymerization by cyclic voltammetry. Within the possible variety, the scan rate is 0.1 V/s, check 10 to 30 circles, obtain and dry to obtain the changed electrode.

( 2) Drawing of standard curve
The modified electrode prepared in step (1) was utilized as the working electrode, the saturated calomel electrode was made use of as the reference electrode, as well as the platinum electrode was made use of as the complementary electrode to develop a three-electrode system, and also the 0.1 mol/L PBS barrier solution with pH= 10 was utilized as the blank remedy for discovery Luminescent strength, the three-electrode system was put in different concentrations of Allura Red (1.0 × 10-7mol/ L, 5.0 × 10-7mol/ L, 1.0 × 10-6mol/ L, 5.0 × 10-6mol/ L, 1.0 × 10-5mol/ L, 5.0 × 10-5mol/ L as well as 1.0 × 104mol/L) PBS buffer remedy of pH= 10, carry out cyclic voltammetry scanning in the possible variety of 0.1 V to 1.5 V, the voltage of photomultiplier tube It is 800V. Under this problem, document the E-ECL contour and also read out the height worth of the luminous strength. After statistics of the acquired information, take the focus of Allura Red Color as the abscissa, and the luminescent intensity difference in between the blank remedy as well as Allura Red as the ordinate, draw a typical curve, and then get the matching direct regression formula. The linear range of the above equation is 1.0 × 10-7 ～ 1.0 × 10-4mol/ L, the size of the glazed carbon electrode made use of according to the here and now innovation is 3mm, as well as the discovery limit is 0.6 × 10-7mol/ L.

( 3) Detection of examples
Select commercially readily available watermelon beverage sample 1.0 mL as well as location it in a 100mL volumetric flask, set the volume to 100mL with a 0.1 mol/L PBS barrier service of pH=10, get 20mL of the gained solution for electrochemiluminescence detection, and also comply with steps (2) the same electrochemical examination technique to evaluate the sample service to be checked to acquire the ECL luminescent strength value and also its difference with the empty remedy luminous strength worth, and also the resulting luminescent intensity value difference is direct regression corresponding to the conventional contour obtained in step (2) The equation computes the focus of Allura Red Shade in the sample to be evaluated.