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Zhishang Chemical

Agarose Le CAS 9012-36-6

Molecular Formula: C10H15N3O3

Formula Weight: 0

ZSpharmac: Agarose Le Supplement

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Product Name: Agarose Le
CAS No: 9012-36-6
Purity: 99%

Basic Info

Product Name:Agarose Le
Other Names:Agarose, Low Melt Point
CAS:9012-36-6
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Pharmaceutical Raw Materials
Appearance:White to Slightly Cream Suspension 
EINECS No.:232-731-8
Storage:2-8°C
Provide:Agarose Le MSDS;
Agarose Le COA

What is Agarose Le?

Agarose Le is a chain-like neutral polysaccharide made up of D-galactose and also 3,6-lactone-L-galactose. key. At low temperature, the agarose sugar chains depend on hydrogen bonding to ensnare each other in a double helix to create a dual helix structure, which is very closely organized to form a gel, which is white and turbid; at high temperature, the hydrogen bonds in the architectural system are damaged, as well as the The random coil is dispersed in water, the solution is clear and transparent, as well as it is a polymer with unique temperature level responsiveness.

Agarose Le Properties:

Melting point ≤90 °C (4% in water)(lit.)
Boiling point 100℃
density 1.00 g/mL at 20 °C
Fp 38 °C
storage temp. 2-8°C
solubility H2O: 20 mg/mL hot, slightly hazy, very faintly yellow
form suspension (75% in water)
color White to slightly cream
OdorOdorless
Water Solubility Soluble in water.

 

Agarose Le Uses

  1. Agarose Le as biochemical reagent.
  2. Agarose Le is used for deoxyribonucleic acid (DNA), lipoprotein and immunoelectrophoresis.  It is made use of for the determination of liver disease B antigen (HAA) in medical medication. Blood electrophoresis analysis. Alpha-fetoglobin assay. Diagnosis of diseases such as liver disease, liver cancer and also cardiovascular diseases.

Agarose Le Preparation

A preparation approach of reduced solidification temperature agarose, consisting of the steps:
( 1) Liquify 4.0 g of agarose powder in 150 mL of deionized water at 90 ° C to prepare an Agarose Le solution;
( 2) After the above-mentioned Agarose Le solution was naturally cooled to 82 ° C., the sodium hydroxide remedy of 0.9 mL of salt borohydride was added thereto to carry out reduction response for 15 min. Throughout the response, the shade change of the service was observed. When the shade transformed from light environment-friendly to colorless and clear When fluid, the reduction reaction is terminated. In the salt hydroxide solution of sodium borohydride, the focus of salt borohydride is 4.4 mol/L, as well as the solvent is 10.0-20.0 mol/L NaOH solution;
( 3) adding 40 mL of sodium hydroxide service with a concentration of 3.8 mol/L to the service obtained in step (2) to accomplish alkalization therapy;
( 4) The remedy of step (3) was quickly cooled to 5 ° C, and also 20-60% (v/v) liquid service of ethylene oxide was slowly included dropwise to the concentration of ethylene oxide in the solution within 60 min while stirring. It is 1.0 ~ 5.0 wt%, as well as the reaction is stirred for 60min;
( 5) heating the solution obtained symphonious (4) to 70 ° C., adding 3.0 mol/L acetic acid option dropwise while mixing to adjust the pH value of the service to 7.2;
( 6) adding warm isopropanol at 45 ° C to the solution symphonious (5) while mixing, and then standing for 1.0-3.0 h for alcohol precipitation category;
( 7) the product gotten in step (6) goes through centrifugal filtration to obtain a white precipitate, crushed after being immersed in the isopropanol of 60% quantity focus, then washed 4 times with isopropanol, as well as pressed and also dehydrated to obtain a filter cake; (8) After the above-mentioned filter cake is positioned in an air drying out oven at 55 ° C as well as dried at a consistent temperature for 12.0 h, then shattered as well as sieved with a 200-mesh filter to obtain the Agarose Le with the reduced solidification temperature. The melting temperature of the agarose is 60.0 ° C, the solidification temperature is 26.5 ° C, and also the gel stamina is 265 g/cm2.

Agarose Le modification method

Agarose Le isolated in nature has a high phase transition temperature, which limits the application of Agarose Le in biomedicine and other fields. According to the temperature response mechanism of Agarose Le, changing the hydrogen bond interaction between the inside/outside of the Agarose Le structural unit and with the water molecules around the structural unit, and regulating the formation of the Agarose Le double helix structure, can effectively reduce its phase transition temperature and improve the Agarose Le. Sugar use performance.
A method for modifying Agarose Le, the method is:
Add 0.1 g of low melting point Agarose Le and 100 mL of deionized water to a round-bottomed flask and heat to fully dissolve the Agarose Le. Cool to 50°C, add 0.5g sodium hydroxide (NaOH) and 1.6mL epichlorohydrin, react at 30°C for 2h, add 5mL containing 0.025g methoxypolyethylene glycol amine (mPEG-NH2) (molecular weight is 20k) deionized water, wherein the mass ratio of Agarose Le and methoxy polyethylene glycol amine is 1:0.25; after reacting at 30 ° C for 72h, the reaction solution is transferred into a dialysis bag, and the molecular weight cut-off of the dialysis bag is 50k , dialyzed for 3 days, changed the water every 12h, and lyophilized. The polymer prepared in this example is: Agarose-g-PEG20k-1.
In this method, through alkalization reaction and nucleophilic substitution reaction, sodium hydroxide and epichlorohydrin are added to low-melting Agarose Le aqueous solution at 30 °C for 2 h, and methoxypolyethylene glycol amine of different quality is added at 30 °C. After 72 hours of reaction, after dialysis and freeze-drying, agarose-graft-polyethylene glycol monomethyl ether polymer (Agarose-g-mPEG) was prepared. The method can destroy the formation of the agarose double helix structure, lower the phase transition temperature of the agarose, and reduce the immune response of the human body to the drug. Moreover, the agarose phase transition temperature is effectively adjusted according to the PEG reaction ratio.

How to purify DNA from Agarose Le

1. According to the protocol of Agarose Le electrophoresis, make the following modifications:
NOTE: Gel purification can use as low a concentration of agarose as possible (in the distinguishable range), so keep in the range of 0.7%-0.8% if possible.
NOTE: Need to get better resolved bands. This can be achieved by using wider gel combs and running the gel at lower voltages.
Note: If you want to reserve more space to cut bands and reduce DNA contamination, you can leave empty lanes between sample-to-sample.
NOTE: To reduce the risk of DNA damage, it is best to limit the time DNA is exposed to UV light.
2. Once the gel runs, the gel needs to be moved to a UV box (ensure proper UV protection – especially for your eyes!), since the plastic blocks most UV rays, it needs to be removed from the gel tray Remove the gel and then use a clean sterile blade to cut the desired DNA fragment from the gel.
NOTE: To protect the UV box, it is a good idea to place the gel on a glass plate if you can. Glass plates will not reduce UV significantly compared to plastic trays, but will protect the UV box from being cut by the blade.
NOTE: Try to remove excess gel around the strip. This is especially important during DNA purification, as many kits cannot handle more than a fixed volume of gel in one system.
3. Place the gel into the labeled EP tube.
4. Weigh with a scale. The system was zeroed with an empty tube, after which the gel was weighed. Alternatively, the weight of the empty tube can be subtracted from the weight of the gel tube. The weight of the gel is proportional to its liquid volume and is used to determine the amount of each buffer added during DNA isolation.
5. Finally, the DNA needs to be separated from the gel. This is available using a commercial gel purification kit. Follow the relevant instructions to complete the experiment.
NOTE: It is often important to determine the concentration of isolated DNA before using gel-purified DNA for further experiments.

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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