1. Natural event All-natural Acido Linoleico Conjugado mostly exists in milk fat and also meat items of rumen animals such as livestock as well as sheep, and also the web content of milk fat per gram arrays from 2 to 25 mg, as well as the content of CLA raises with the age of cows. The formation of CLA is because of the conversion of linoleic acid to CLA by the anaerobic butyrifibriolyticus linoleic acid isomerase in the gut of ruminants, mostly to the naturally active cis-9, trans-11 isomer. CLA is likewise existing in percentages in the cells, blood and also body fluids of various other pets. CLA is likewise discovered in human milk.
2. Fabricated synthesis CLA can be synthesized by base-catalyzed isomerization reaction with linoleic acid or vegetable oil rich in linoleic acid as substrate. The initially artificially manufactured Acido Linoleico Conjugado is a mix of different isomers, primarily having cis-trans, trans-cis isomers and also a percentage of cis-cis, trans-trans isomers. The improved synthesis procedure can make CLA more purified, the content of cis-9, trans-11 as well as tRNAs-10, cis-12 isomers exceeds 50%, and the non-CLA parts are less than 1%. Currently, man-made synthesis primarily has the complying with artificial approaches.
( 1) Alkaline isomerization of linoleic acid utilizes natural grease as resources, under the problems of KOH as catalyst as well as propylene glycol as solvent, mixed and refluxed in the presence of nitrogen security and also heating, after reflux, include aqueous solution, and adjust the pH worth with hydrochloric acid, drawn out with n-hexane, the essence was washed with brine, dried with magnesium sulfate, as well as finally concentrated under reduced pressure to obtain a combination of conjugated linoleic acid. This alkaline isomerization approach has a basic process, and also the gotten product is safe and also simple to take care of, so it is widely utilized and also appropriate for the production of conjugated linoleic acid from veggie oils abundant in linoleic acid, such as soybean oil, red Flower oil, cottonseed oil, corn oil, etc, as well as their products can be widely used in food processing.
( 2) Dehydration of oleic acid allyl alcohol Allyl oleic acid is oleic acid generated peroxide by light, as well as after being reduced, 8-hydroxyl or 11-hydroxyl oleic acid allyl alcohol is obtained. Allyl oleic acid is an additional item of oleic acid, which exists in natural foods as well as can be dried out into conjugated linoleic acid under acid-catalyzed (BF3, HCl) conditions. The methylation of conjugated linoleic acid was carried out by using the specificity of G. candidum lipase. Because the lipase has specificity for cis-9, trans-11-octadecadienoic acid, it can be executed Methyl esterification has no result on trans-10, cis-12-octadecadienoic acid, and also molecular distillation innovation can get cis-9, trans-11-octadecadienoic acid with greater pureness.
( 3) Esterification technique Castor oil is dissolved in dichloromethane as well as methanol option, as well as esterification reaction takes place under the condition of salt as well as reflux to produce ricinoleic acid methyl ester. The apart as well as cleansed ricinoleic acid methyl ester can react with 12-methylsulfonyl chloride to generate 12-methylsulfoxymethyl oleate, as well as the toluene option of 12-methylsulfoxymethyl oleate remains in DBU (1,8 -diazabicyclo [5.4.0] undecane-7-ene) under the catalysis of reflux, the total digestion product gotten is generally cis-9, trans-11-octadecadienoic acid methyl ester. Acido Linoleico Conjugado items with higher purity can be obtained by using urea for splitting up and also purification.
( 4) Microbial fermentation Microbial fermentation is making use of linoleic acid isomerase in bacteria to transform linoleic acid right into conjugated linoleic acid. From the research study results that have been acquired until now, lactic acid microorganisms are optimal stress, especially acidophilic lactic acid bacteria can successfully convert linoleic acid or glycerol linoleate into conjugated linoleic acid. Lactic acid germs include linoleic acid isomerase, and its action site is the C12 double bond of fat as opposed to the C9 double bond, to make sure that it can convert linoleic acid right into cis-9, trans-11-octadecadienoic acid, yet not various other isomers, the gotten Acido Linoleico Conjugado can be straight extracted from the cell culture fluid.