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Acid Red 27 CAS 915-67-3

Molecular Formula: C20H11N2Na3O10S3

Formula Weight: 604.47

ZSpharmac: Acid Red 27 Supplier

Product Name: Acid Red 27
CAS No: 915-67-3
Purity: 99%

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Basic Info

Product Name:Acid Red 27
Other Names:Food Red No. 2
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Dark Reddish-Brown to Dark Brown Crystalline Powder
EINECS No.:213-022-2
Storage:Sealed in dry,Room Temperature
Provide:Acid Red 27 MSDS;
Acid Red 27 COA

What is Acid Red 27?

Acid Red 27 is a natural pigment extracted from amaranth, a water-soluble azo red dye, diazotized by 1-naphthylamine-4-sulfonic acid and 2-naphthol-3,6-disulfonic acid Coupled made. Its bright colors and high safety. The pigment is stable to most food additives, so it can be used for coloring beverages, candies, cakes, wines, etc., making it colorful and attractive, with a sweet and sour taste. It can also be used as the coloring of medicines (sugar coating, capsules), health care products and cosmetics.

Acid Red 27 Property

Melting point >300°C
density 1.5
storage temp. Sealed in dry,Room Temperature
solubility H2O: soluble10mg/mL
form Crystalline Powder
Colour Index 16185
color Dark reddish-brown to dark brown
Water Solubility 50 g/L (20 ºC)


Acid Red 27 Uses

  1. Acid Red 27 is used as a food coloring agent. It can be used in red and green silk and dyed canned cherries (for decoration) according to the regulations of our country. The maximum usage amount is 0.05g/kg in the package, green plum, hawthorn products and pickled vegetables.
  2. Acid Red 27 is used as redox indicator, such as indicator for titration of trivalent arsenic, antimony and hydrazine; food, drug and cosmetic coloring, cell staining in tissue culture; color photomicrograph; wool and silk dyeing.

Extraction Method of Acid Red 27

1. Solid Phase Extraction (SPE)
Solid-phase extraction is a common method for extracting synthetic pigments in food. It can eliminate interfering components and only extract pure substances containing target analytes. The operation is quick and easy. SPE consists of 4 steps: first deairing and activating the ligands on the surface of the extraction particle; loading the sample; washing to remove interfering components that do not contain the analyte of interest; and finally elution to release the analyte of interest from the sorbent thing. The dispersive solid-phase extraction enrichment method is a continuation of the solid-phase extraction method, and is an extraction method that uses polydopamine-coated iron ferric oxide nanoparticles as an extraction agent. The use of nanomaterials makes the sample recovery rate as high as 91.9%, which has the remarkable advantages of high sensitivity and good repeatability. Acid Red 27 was extracted from beverages with C18 solid phase extraction kit and 18% isopropanol, and the recovery rate reached 81%.

2. Liquid-liquid extraction (LLE)
Liquid-liquid extraction (LLE) is called solvent extraction, which uses two immiscible liquids to separate the difference in solubility of the same compound. Acid Red 27 was extracted from carbonated beverages, alcoholic beverages, syrups, ice cream and chocolate using liquid-liquid extraction with a recovery rate of 90.9%. Using a two-phase aqueous system, using ionic liquids for micro-extraction, the separation efficiency is greatly improved.

3. Membrane separation
Membrane is a thin layer of semi-permeable material with filtering properties, which can remove interfering substances in food and drink to a certain extent. Using membrane filtration to extract Acid Red 27 in the beverage sample, the filtered beverage sample has less impurities and is obviously easier to carry out subsequent processing.

4. Ultrasonic extraction method
Ultrasonic extraction, with its unique advantages of low extraction temperature, high extraction rate and short extraction time, has been used in the extraction of effective content of Chinese medicinal materials and various animals and plants in recent years. High-tech means. The dried and pulverized red amaranth is taken according to different solid-liquid ratios and corresponding distilled water is added as an extraction solvent, and ultrasonic extraction is carried out according to various conditions (liquid-solid ratio, ultrasonic power, temperature, time). After the extraction, filter with suction, take the red transparent filtrate and place it in a vacuum rotary evaporator to concentrate, then pour the concentrate into an evaporating dish, and place it on a water bath to dry. The water temperature is controlled at about 50°C, and the resulting red paste is the red pigment.

5. Microwave extraction method
Microwave extraction is a newly developed new technology that uses microwave energy to increase the extraction rate. At present, in addition to being mainly used for environmental sample pretreatment, it is also used in fields such as biochemistry, food, industrial analysis and natural product extraction. The method is: take a certain amount of pretreated amaranth leaves, use a buffer solution of pH=5.0 as the extractant, carry out microwave extraction with a microwave extraction apparatus, set the microwave extraction conditions as: temperature 40 ° C, pressure 5 standard atmospheres, power 500W. After the reaction was completed, it was cooled to room temperature, filtered, concentrated under reduced pressure by a rotary evaporator at 50°C to form a dark red extract, dried in vacuum and weighed. Compared with the same extractant and non-microwave extraction method, the microwave extraction rate is high and the extraction time is greatly shortened. In the extraction process of natural pigments, microwave extraction technology has high promotion value.

6. Ultrafiltration-resin method
Ultrafiltration is a pressurized membrane separation technology. Membrane separation technology is a new separation and purification method. It is easy to operate, fast in separation speed, good in effect, pollution-free, and low in cost. It has been widely used in food industry, medicine and Industrial separation and extraction process. The process flow is: fresh amaranth (soaked in acidic water with pH=2 for 24h→extract solution→microfiltration→ultrafiltration→resin→eluent concentration→vacuum drying→pigment product. The microfiltration in this method can effectively remove the crude extract The suspended solids and some polysaccharides and pectin molecules are helpful for the subsequent ultrafiltration purification and shorten the ultrafiltration time. The ultrafiltration-resin combination technology has simple process, easy operation, no pollution, and low production cost, and is suitable for separating and extracting Acid Production of Red 27 pigment.

Analysis Method of Acid Red 27

1. High Performance Liquid Chromatography (HPLC)

High performance liquid chromatography is commonly used to analyze pigments. Most HPLCs are equipped with UV-Vis, diode array, or photodiode array detectors. There are various mobile phases and stationary phases. The commonly used separation columns are octadecyl column and monomer octyl column. The detection limit and quantification limit of Acid Red 27 in carbonated beverages, cocktails, candies, jelly and ice cream determined by HPLC-spectrophotometry were 6.4 and 19 ng/mL, respectively. The ultra-high performance liquid chromatography derived on the basis of high performance liquid chromatography not only has higher analysis accuracy, but also greatly shortens the analysis time. The content of amaranth in meat products was determined by ultra-high performance liquid chromatography, and the detection limit was 0.02mg/kg, and the quantification limit was 0.05mg/kg.

2. Liquid chromatography-mass spectrometry

Liquid chromatography-mass spectrometry is one of the chromatographic techniques used to determine pigments in food. Its principle is the same as that of ordinary high performance liquid chromatography, and it is necessary to select a suitable mobile phase and stationary phase. But mass spectrometers can overcome spectral interference and make measurements more accurate. The chromatographic analysis method based on liquid chromatography-mass spectrometry was used to determine Acid Red 27 in ice cream, chocolate and candy, and the detection limit and quantification limit were 5 and 10 mg/kg, respectively.

3. TLC

Thin-layer chromatography is another basic chromatographic method that screens for unknown compounds in food by comparing the ratio shift and color of a sample to a standard. Thin-layer chromatography has a relatively small sample load, and generally uses silica gel as the stationary phase, and the mobile phase is selected according to the difference in sample properties. The determination of Acid Red 27 in beverages by thin-layer chromatography has a lower detection limit than high-performance liquid chromatography, and its sensitivity is not outstanding enough.

4. Electrochemical sensor

Electrochemical sensors have been widely used in the fields of proteomics, biochemistry, drug and food analysis due to their advantages of high sensitivity, easy operation, good selectivity, and easy miniaturization. In electrochemical systems, the key point is to design and modify highly sensitive and selective working electrodes. An electrochemical sensor platform based on graphene nanosheets to detect the content of amaranth in beverages. Ultrasonic exfoliation of graphite powder in N-methylpyrrolidone broadens the linear range from 2.5nm to 125nm. In the electrochemical system, the oxidation reaction between different RF polymers and Acid Red 27 showed different electrochemical activities, and this new sensing system has a good application prospect in monitoring synthetic pigments.

5. Molecularly imprinted polymers

Molecularly imprinted polymers
It is a biomimetic synthetic receptor with a specific cavity designed for the target molecule. Molecular polymers can not only specifically bind target molecules, but also participate in the composition of optical fiber sensors, which are widely used in environmental analytical chemistry, clinical analysis, and food. A newly developed molecularly imprinted electrochemical sensor uses a composite material induced by a magnetic field to self-assemble redox graphene and ferroferric oxide. In the molecular imprinting technique, the complex includes the functional monomer of aniline, Acid Red 27 sample and ferric oxide, which are pre-placed on the template through p-p stacking and hydrogen bond self-assembly, and then polymerized under magnetic field induction.

6. Capillary Electrophoresis

Electrophoresis is the general name for a family of separation techniques involving the application of an electric field in a capillary. According to different separation modes, capillary electrophoresis can be divided into capillary zone electrophoresis, capillary micellar electrokinetic chromatography, and capillary isotachophoresis. Due to the narrow inner diameter of the capillary, the capillary electrophoresis technique has a very low sample capacity, resulting in a low detection limit for the analyte. However, this method has achieved good results in the detection of amaranth in beverages and ice cream. Capillary zone electrophoresis combined with PDA detector was used to determine the content of Acid Red 27 in the beverage. At the wavelength of 280nm, the detection limit and quantification limit of Acid Red 27 were 1.1 and 3.61 mg/mL, respectively.

About Products:
If you need Acid Red 27 as a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced manufacturer and supplier of Acid Red 27, ZSpharmac will provide Acid Red 27 raw materials for customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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