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Acid Hydrolyzed Casein CAS 65072-00-6

Molecular Formula: C21H41N5O11

Formula Weight: 539.57714

ZSpharmac: Acid Hydrolyzed Casein Supplier

Product Name: Acid Hydrolyzed Casein
CAS No: 65072-00-6
Purity: 99%

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Basic Info

Product Name:Acid Hydrolyzed Casein
Other Names:N-Z-AMINE A
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Off-White to Beige Powder
EINECS No.:265-363-1
Storage:+15C to +30C
Provide:Acid Hydrolyzed Casein MSDS;
Acid Hydrolyzed Casein COA

What is Acid Hydrolyzed Casein?

Acid hydrolyzed casein is a white or light yellow powder made from top quality casein as raw material, deeply hydrolyzed with solid acid, decolorized, desalted, focused, and also furthermore spray-dried. It is straightforward to absorb dampness, soluble in water, as well as has a sauce flavor. It is an acidic decomposition product of casein, which can be broken down for amino acids. Acid hydrolyzed casein is a product prepared from casein in addition to its appropriate products with strong acid hydrolysis, decolorization, neutralization, desalination, drying out and other procedures, the main parts of which are amino acids as well as also brief peptides. According to item purity (chloride web material), acid hydrolyzed casein is primarily separated into two types: industrial high quality (chloride content greater than 3%) as well as likewise pharmaceutical quality (chloride material less than 3%).

Acid Hydrolyzed Casein Property

Melting point 280 °C
RTECS FI3519450
storage temp. +15C to +30C
solubility 310g/l
form Off-white to beige powder
color Off-White to beige
PH4.7-5.7 (50g/l, H2O, 20℃)
Water Solubility Soluble in water.


Acid Hydrolyzed Casein Uses

1. In the pharmaceutical sector, acid hydrolyzed casein is usually made use of as a basic material for pharmaceutical items.
2. In the food field, acid hydrolyzed casein is mostly made use of as an additive for premium nutritional products.
3. In the everyday chemical market, acid hydrolyzed casein can be utilized as the raw item of amino acid skin care items together with amino acid everyday chemical products;
4. In the fermentation market, acid hydrolyzed casein is primarily used for microbial society gadget;
5. In the feed market, acid hydrolyzed casein can be utilized as a feed additive to increase the nutrients of relative animal canine feed; in the plant fertilizer market, acid hydrolyzed casein is a vital resources for amino acid steel chelated plant foods. It can be seen that acid hydrolyzed casein has a truly range of uses.

Application of Acid Hydrolyzed Casein

CN201010122050.9 divulges an approach for preparing resveratrol-producing cells and its special suspension tool. The unique suspension medium of today innovation is a medium gotten by including the complying with materials to B5 fundamental society tool or NN1969 culture tool: acid hydrolyzed casein, NAA, KT, BA and carbon resource; where the final concentration of acid hydrolyzed casein is 0.015-0.030 g/100ml, and also the final concentration of NAA is 0.01-1.0 0mg/L, the final concentration of KT is 0.1-2.00 mg/L, and the final concentration of bachelor’s degree is 0.05-1.00 mg/L. In the innovation, the explants are first of all generated to callus; then the callus is moved to the expansion tool for society; and after that the grape genotype with high resveratrol production is determined by the induction method of ultraviolet radiation. The organ types with high resveratrol manufacturing were then recognized. Lastly, through suspension culture, a great deal of cells are broadened and caused, and also ultimately high-yielding resveratrol cells are obtained.

CN201010190788.9 divulges a spore-producing culture medium for Pyramid rot of guava, which contains oats, sugar, acid hydrolyzed casein and guava fruit juice. Glucose was used as the carbon resource and also acid hydrolyzed casein was utilized as the nitrogen resource to offer the carbon as well as nitrogen elements required for sporulation of the microorganisms; the added growth factor– vitamins in oats was needed for the typical metabolic process of microorganisms; as well as met the dietary demands of the germs. The spore manufacturing medium of guava blister rot fungi of the here and now creation has a spore return of 4 × 103 spores/25 milliliters of society tool after being grown for 7 days.

Preparation of Acid Hydrolyzed Casein

A pharmaceutical grade hydrolyzed casein and its preparation method:

(1) Mix casein and concentrated hydrochloric acid in an autoclave at a mass/volume ratio (g/mL) of 1:0.6~1:1;

(2) Adjust the pressure of the kettle to 200-300kPa, the temperature to 100-120°C, heat and hydrolyze for 2-6 hours, and steam condenses and refluxes during the hydrolysis process;

(3) Release the pressure after hydrolysis, change the reflux device to a distillation device, raise the temperature to 180~220°C, and heat

(4) After cooling the reactor, open it, add activated carbon at a mass ratio of activated carbon to casein of 1-2:1, add water at a mass ratio of water to casein of 8-16:1, adjust the pressure of the kettle to 150-250kPa, the temperature to 100-120°C, heat for 2-4 hours for decolorization, and condense and reflux the steam during the decolorization process;

(5) After cooling down to room temperature after depressurization, perform suction filtration operation on the mixture in the kettle, and collect the filtrate;

(6) Weigh the anion exchange resin according to the ratio of casein to resin mass ratio of 1:10 to 1:20, fill it in an ion exchange column, rinse with distilled water to remove impurities; add 0.5 mol/L HCl solution 4 times the mass of the resin to soak for 2 hours, wash with distilled water until neutral; then add 0.5 mol/L NaOH solution 4 times the mass of the resin to soak for 2 hours, wash with distilled water until neutral, and set aside;

(7) Use a peristaltic pump to transport the filtrate to the upper end of the ion exchange column at a flow rate of 2.0-6.0 mL/min, and collect the effluent at the lower end of the column;

(8) Place the effluent in a steel tray for freeze-drying at a temperature of -50°C, a pressure of 500-600Pa, and a time of 24 hours. The obtained powdery product is pharmaceutical grade hydrolyzed casein.

Study on Biological Activity of Acid Hydrolyzed Casein

Bai Guijie et al. explored the effects of different acid-hydrolyzed casein on the capsular polysaccharide production of W135 and Y Neisseria meningitidis (meningococcus). Methods The improved semi-comprehensive high-salt medium and low-salt medium were prepared with acid-hydrolyzed casein with NaCl mass fraction of 37% and 14% as organic nitrogen source, respectively, using fully automatic bacterial fermenter to culture W135 and Y meningococci in the two mediums, and comparing the growth time of these two strains in the two mediums, the bacterial density of the harvest liquid (A600 nm value), the capsular polysaccharide content in the supernatant of the harvest liquid after removal of bacterial cells and complex polysaccharides, and the yield of refined sugar; Effects of different final contents of cetyltrimethylammonium bromide (CTAB) solutions in high-salt medium harvest liquid and its 2-fold dilution on polysaccharide precipitation.

Results There was no statistically significant difference in the culture time between W135 group and Y group meningococcus in the two media (P>0.05), but the bacterial density of the high-salt medium harvest liquid and the polysaccharide content in the supernatant after removing bacteria were higher than those of the low-salt medium harvest liquid, and the difference was statistically significant (P<0.05).

After 2-fold dilution of the high-salt culture solution, when the final volume fraction of CTAB was 0.04%, the polysaccharides of group W135 and group Y meningococci were all precipitated, while the undiluted high-salt culture solution could not completely precipitate the polysaccharides of group W135 and group Y meningococcus even if the final volume fraction of CTAB increased to 0.14%. Conclusion Different acid-hydrolyzed casein can affect the growth density and capsular polysaccharide production of W135 and Y meningococci, and it is necessary to control the salt content of the harvest liquid when using CTAB solution to precipitate capsular polysaccharide.

About Products:
If you need Acid Hydrolyzed Casein as a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced manufacturer and supplier of Acid Hydrolyzed Casein, ZSpharmac will provide Acid Hydrolyzed Casein raw materials for customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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