Zhishang Chemical

Welcome to Zhishang Chemical      +86-176 5311 3209     Simon@sdzschem.com

Zhishang Chemical

Acid Hydrolyzed Casein CAS 65072-00-6

Molecular Formula: C21H41N5O11

Formula Weight: 539.57714

ZSpharmac: Acid Hydrolyzed Casein Supplier

Product Name: Acid Hydrolyzed Casein
CAS No: 65072-00-6
Purity: 99%

Navigation Bar

Basic Info

Product Name:Acid Hydrolyzed Casein
Other Names:N-Z-AMINE A
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Off-White to Beige Powder
EINECS No.:265-363-1
Storage:+15C to +30C
Provide:Acid Hydrolyzed Casein MSDS;
Acid Hydrolyzed Casein COA

What is Acid Hydrolyzed Casein?

Acid Hydrolyzed Casein is a white or light yellow powder made from premium casein, which is deeply hydrolyzed with solid acid, decolorized, desalted, concentrated, as well as spray-dried. It is very easy to absorb dampness, soluble in water, as well as also has a sauce taste. It is an acidic decay item of casein, which can be degenerated for amino acids. Acid hydrolyzed casein is an item prepared from casein and its relevant products with solid acid hydrolysis, decolorization, neutralization, desalination, drying and various other processes, the primary components of which are amino acids and also short peptides. According to item pureness (chloride content), acid hydrolyzed casein is mostly separated into 2 types: industrial quality (chloride content more than 3%) and also pharmaceutical quality (chloride content less than 3%).

Acid Hydrolyzed Casein Property

Melting point 280 °C
RTECS FI3519450
storage temp. +15C to +30C
solubility 310g/l
form Off-white to beige powder
color Off-White to beige
PH4.7-5.7 (50g/l, H2O, 20℃)
Water Solubility Soluble in water.


Application of Acid Hydrolyzed Casein

In the pharmaceutical market, acid hydrolyzed casein is mainly used as a basic material for pharmaceutical items, such as nuclear casein dental liquid, amino acid metal chelation preparations; such as a nutritional make-up consisting of whey healthy protein and also protein source of hydrolyzed casein protein, such as formula for early infants. The proportion of whey healthy protein to hydrolyzed casein might be from concerning 90:10 to about 50:50 by weight. The degree of hydrolysis of the hydrolyzed casein can vary from about 20% to about 80%. In addition, approaches for enhancing stomach draining as well as enhancing digestibility in a target subject (eg, a premature infant) are offered. When carried out, the dietary structure can advertise digestive propulsion as well as absorbent capacity. Hence, much better growth and also advancement, especially neurodevelopment, of the target subject can be achieved.

In the food market, acid hydrolyzed casein is generally made use of as an additive for premium dietary products to supplement amino acids; as an example, to prepare a low-sensitivity newborn formula milk powder, the raw materials consist of raw milk, lactose, α-whey healthy protein, and also hydrolyzed whey protein, hydrolyzed casein, naturally active compounds, as well as also divulges an approach for preparing the milk powder, consisting of (1) mixing the raw materials with water and also entirely liquifying them to get feed liquid A, as well as the raw materials include raw milk, lactose, and α-whey protein, hydrolyzed whey protein, and also hydrolyzed casein; (2) homogenize, disinfect, focus, as well as spray-dry the product fluid An obtained symphonious (1) to get material B; (3) integrate the naturally active substances in the raw materials with the product B is blended evenly in the dry blending workshop to obtain low-sensitivity newborn formula milk powder. The invention has the advantages of thinking about hyposensitivity and enhancing immunity, addressing the trouble of infant allergic reaction by decreasing irritants and improving resistance; the preparation procedure is easy, the milk powder has excellent solubility, secure service life, as well as is difficult to oxidize.

In the daily chemical industry, acid hydrolyzed casein can be used as a raw material for amino acid skin care items and also amino acid everyday chemical items;

In the fermentation sector, acid hydrolyzed casein is mainly made use of in microbial culture media;
In the feed market, acid hydrolyzed casein can be utilized as a feed additive to strengthen the nutrition of pet feed; in the plant fertilizer sector, acid hydrolyzed casein is a vital raw material for amino acid steel chelated plant foods. It can be seen that acid hydrolyzed casein has a very vast array of uses.

Preparation of Acid Hydrolyzed Casein

A pharmaceutical grade hydrolyzed casein and its preparation method:

(1) Mix casein and concentrated hydrochloric acid in an autoclave at a mass/volume ratio (g/mL) of 1:0.6~1:1;

(2) Adjust the pressure of the kettle to 200-300kPa, the temperature to 100-120°C, heat and hydrolyze for 2-6 hours, and steam condenses and refluxes during the hydrolysis process;

(3) Release the pressure after hydrolysis, change the reflux device to a distillation device, raise the temperature to 180~220°C, and heat

(4) After cooling the reactor, open it, add activated carbon at a ratio of 1-2:1 mass ratio of activated carbon to casein, add water at a ratio of 8-16:1 mass ratio of water to casein, and adjust the kettle pressure to 150~250kPa, temperature 100~120℃, heat decolorization for 2~4h, steam condenses and refluxes during the decolorization process;

(5) After cooling down to room temperature after depressurization, perform suction filtration operation on the mixture in the kettle, and collect the filtrate;

(6) Weigh the anion exchange resin according to the mass ratio of casein to resin in the ratio of 1:10 to 1:20, fill it in the ion exchange column, wash with distilled water to remove impurities; add 0.5mol/ Soak in LHCl solution for 2 hours, wash with distilled water until neutral; then add 0.5mol/L NaOH solution 4 times the mass of the resin to soak for 2 hours, wash with distilled water until neutral, and set aside;

(7) Use a peristaltic pump to transport the filtrate to the upper end of the ion exchange column at a flow rate of 2.0-6.0 mL/min, and collect the effluent at the lower end of the column;

(8) Place the effluent in a steel tray for freeze-drying at a temperature of -50°C, a pressure of 500-600Pa, and a time of 24 hours. The obtained powdery product is pharmaceutical grade hydrolyzed casein.

Study on Biological Activity of Acid Hydrolyzed Casein

Bai Guijie et al. explored the effects of different acid-hydrolyzed casein on the capsular polysaccharide production of W135 and Y Neisseria meningitidis (meningococcus). Methods The acid hydrolyzed casein with NaCl mass fraction of 37% and 14% was used as organic nitrogen source to prepare improved semi-comprehensive high-salt medium and low-salt medium. Group Y and group Y meningococci, and compared the growth time of these two strains in the two media, the bacterial density of the harvest liquid (A600 nm value), and the capsules in the supernatant of the harvest liquid after removal of bacterial cells and after removal of complex polysaccharides Polysaccharide content, and refined sugar yield after purification; compare the effects of cetyltrimethylammonium bromide (CTAB) solutions with different final contents in the high-salt medium harvest solution and its 2-fold dilution on the precipitation effect of polysaccharides influences.

Results There was no significant difference in the culture time of W135 group and Y group meningococcus in the two media (P>0.05), but the bacterial density of the harvest liquid of the high-salt medium and the supernatant of the harvest liquid after removing bacteria The content of polysaccharides in the harvest liquid of high-salt medium was higher than that of low-salt medium, and the difference was statistically significant (P<0.05). <0.05).

After 2-fold dilution of the high-salt culture solution, when the final volume fraction of CTAB was 0.04%, the polysaccharides of group W135 and group Y meningococcus were all precipitated, while the undiluted high-salt culture solution even if the final volume fraction of CTAB was increased to 0.14%, still remained unchanged. The W135 and Y meningococcal polysaccharides could not be completely precipitated. Conclusion Different acid-hydrolyzed casein can affect the growth density and capsular polysaccharide production of W135 and Y meningococci, and it is necessary to control the salt content of the harvest liquid when using CTAB solution to precipitate capsular polysaccharide.

About Products:
If you need Acid Hydrolyzed Casein as a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced manufacturer and supplier of Acid Hydrolyzed Casein, ZSpharmac will provide Acid Hydrolyzed Casein raw materials for customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

Get Acid Hydrolyzed Casein Quotation