At present, the latest official diagnosis and treatment plan points out that “the understanding of the physicochemical properties of coronavirus mostly comes from the research on SARS-CoV and MERS-CoV”.
The International Committee on Taxonomy of Viruses officially named the new coronavirus “SARS-CoV-2”, and its genetic characteristics are significantly different from SARSr-CoV and MERSr-CoV. Current research shows that SARS-CoV-2 is more than 85% homologous to bat SARS-like coronavirus (bat-SL-CoVZC45). Previous genomic studies have shown that SARS-CoV is unique in that it contains both mammalian and avian ancestors, and the impact of this recombination in humans has been disastrous.
Although 4-Chloro-3,5-Dimethylphenol has been widely used over the years, its mode of action has not been well studied.
The author wants to start from the mechanism of action of disinfectants to see if it is possible that 4-Chloro-3,5-Dimethylphenol can kill the virus. It seems that there is no way!
Look around and find articles with two alternative experimental studies.
If you don’t want to understand the experimental process, you can directly see the conclusion
In 2005, Wolff et al. used coronavirus 229E to test the antiviral activity of 4.8% 4-Chloro-3,5-Dimethylphenol.
Note: Human coronavirus 229E (HCoV-229E) is a single-stranded positive-stranded RNA virus belonging to the family Coronaviridae, genus Alphacoronavirus, which is one of the viruses that cause the common cold.
experimental method
The 4.8% p-chloro-meta-xylenol stock solution is diluted at 1:40 (v/v) to be tested. At room temperature (23±2°C), prepare several (not mentioned in the text) stainless steel discs (0.7 mm thick, 1 cm in diameter) as carriers in a glass dish as shown in the figure below, and drop each of them on it first. 10 microliters of virus suspension, let it dry for 1 hour, and then dropwise add 50 microliters of the disinfectant to be tested, p-chlorometaxylenol. After 10 minutes of action, the 229E coronavirus was significantly reduced.
The same test method, 75% ethanol for 5 minutes can significantly reduce the virus. It can be seen that the antiviral activity of p-chlorometaxylenol is not superior to that of alcohol.
In 2009, Christine Dellanno et al. used mouse hepatitis virus (MHV-1) to test the antiviral activity of 4.8% 4-Chloro-3,5-Dimethylphenol. The stock solution was diluted 1:40 (v/v) to be tested.
Note: Mouse hepatitis virus (MHV) is a single-stranded positive-stranded RNA virus belonging to the family Coronaviridae and genus Betacoronavirus. Can cause epidemic mouse disease, clinical manifestations of hepatitis, encephalitis and enteritis, high mortality. Before the discovery of SARS-CoV, mouse hepatitis virus was the most well-studied coronavirus, whether in vivo, in vitro or at the molecular level, and an alternative to SARS-CoV.