It is popular that taking in moderate amounts of fruits and vegetables can enhance physical health and wellness and physical function. A healthy and balanced diet regimen, including intake of vegetables and fruits, can alter the gut microbiota. This research study intended to demonstrate the efficiency of created fruit and vegetable supplements (FVS) in regulating antioxidant capability and also intestine microbiota make-up. We hired 30 healthy volunteer topics, matched for age, sex, BMI and cigarette smoking practices, and also randomized them into FVS and also placebo (PLA) teams. Amongst product vitamins, folic acid levels were considerably greater in the FVS group than in the PLA group (p = 0.001), while vitamin B2 degrees were considerably higher in the PLA team than in the FVS team (p = 0.028). Antioxidant capacity measured making use of the oxygen radical absorbance capability (ORAC) method was likewise a little greater in the FVS group than in the PLA group, yet did not get to statistical significance. Nutritional intake examined by 24-h recall did disappoint any kind of significant change after supplementation in either group. Digestive tract microbiome composition gauged by 16S rDNA sequencing showed no distinction in alpha and beta variety, whereas LEfse evaluation revealed adjustments in microbiota after therapy, with lowered wealth of Ruminococcus in the Lachnospiraceae category (p = 0.009), along with those from Erysipelotrichaceae The unclassified category (UC36, p=0.003) of the FVS team compared with the PLA team (verified by SIAMCAT analysis, AUC = 74.1%). The genus Faecalibacterium and also unidentified genera and also families from the order Lactobacillus (UC31) were likewise enhanced in the FVS group compared to the PLA group (p = 0.0474, p = 0.0352, specifically). SCFA measured by gas chromatography-mass spectrometry disclosed elevated levels of 2-methylbutyrate in the FVS group compared to the PLA team (p = 0.0385). Finally, Spearman relationship evaluation showed that in the FVS group, the Faecalibacter category was favorably associated with 2-Methyl Butyric Acid (p = 0.040). In the PLA group, none of the considerable germs correlated with SCFA or product biomarkers. Network analysis validated a favorable connection between the Faecalibacterium genus and also 2-Methyl Butyric Acid. We can wrap up that FVS in healthy individuals changes intestine microbiota composition and metabolites, which may add to minimized pro-inflammatory responses as well as antioxidant ability.