Drawn out from plants
It can be extracted from carrots, peppers or silkworm sand with organic solvents such as petroleum ether. The unsaponifiable matter in the fuel layer when removing chlorophyll is mixed and cleaned once with an equal amount of 1% sodium chloride solution to remove soluble lipid substances, left to stand and divided into layers to get a gasoline layer, which is then distilled under decreased stress to recoup the fuel to get a yellow waxy residue., liquify with oil ether, and swiftly filter; the filtrate travels through a triggered alumina chromatography column, and is eluted with a mixed solution prepared with a volume ratio of oil ether: acetone = 8:2. After vacuum cleaner purification to recuperate the solvent, unrefined oily carotene is acquired, and afterwards Recrystallize with anthracene/ethanol to acquire the item.
Extracted from salina
Salina is an aquatic plankton that expands in salt areas. It is a single-cell algae that can replicate in multitudes under synthetically controlled conditions. There are 2 procedure paths as adheres to.
The petroleum ether extraction process uses 6 # solvent oil to essence damp salt algae powder for about 20 minutes at 50 ° C. After filtration, the filter residue is extracted twice. The filtrate is focused by thin film evaporation, crystallized, filtered, and vacuum cleaner dried to get the finished product. The extraction rate is about 80%.
The grease extraction process involves stirring and extracting grease twice as long as completely dry salt algae powder at 75 ° C for 30 to 60 mins, centrifuging it with a three-legged centrifuge, and then centrifuging it with a disc centrifuge to work out the oil service of Beta-Carotene Powder.
Prep work by fermentation
Usage wort with a family member density of 1.06 to 1.07 as the culture medium, inoculate 2% to 3% red yeast after high temperature sterilization, and society it under air flow at 26 ° C and a Ph value of 5 to 6 for two days. The fermentation brew was divided by high-speed centrifugation for 15 min, washed with distilled water, and dried at 50 ° C to acquire completely dry yeast cells. First treat the stem cells with 2 ~ 3mo1/L hydrochloric acid, then boil them in boiling water for 2 ~ 3 mins, separate by high-speed centrifugation, and clean two times with water to obtain cell fragments. Saturate the dried out cell pieces in acetone, and afterwards centrifuge at broadband to remove the cell pieces to obtain a beta-carotene essence. Concentrate under lowered stress with a rotating slim film evaporator, and remove with chloroform to obtain a Beta-Carotene Powder concentrate. Lastly, concentrate under reduced pressure and spray-dry to get the completed product.
The Grignard reaction synthesis approach uses β-ionone as basic material to acquire β-C14, β-C16, and β-C19 aldehydes successively, and afterwards condenses two particles of β-C19 aldehyde into β-C40 diphenol via Grignard response. After dehydration, The product is acquired after hydrogenation, with a complete yield of 21% based on β-ionone. It can likewise be manufactured by Wittig response, making use of β-ionone as resources, through vinyl-β-ionol, to manufacture the item in the C15+C10+C15 technique. Based on C15 enol, the return is 25%.